Sino Biological/Human cytomegalovirus (HCMV) Glycoprotein B / gB Protein (ECD, Fc Tag)/1/10202-V02H2
商品编号：
10202-V02H2
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Human cytomegalovirus (HCMV) Glycoprotein B / gB Protein (ECD, Fc Tag): Product Information
Purity
> 80 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Lys 700) of human CMV gB (AAA45920.1, with furin cleavage site mutated from "RTKR" to "TTQT") precursor was fused with the Fc region of human IgG1 at the C-terminus.
Accession#
AAA45920.1
Expressed Host
HEK293 Cells
Species
CMV
Predicted N Terminal
Asn 25
Molecule Mass
The recombinant human CMV glycoprotein B ECD/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 914 amino acids and has a predicted molecular mass of 104 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the monomer is approximately 135-145 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human cytomegalovirus (HCMV) Glycoprotein B / gB Protein (ECD, Fc Tag): Images
CMV Glycoprotein B Background Information
Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.
References
- Lopper M,et al. (2002). Disulfide bond configuration of human cytomegalovirus glycoprotein B. J Virol. 76(12): 6073-82.
- Isaacson MK, et al. (2009) Human cytomegalovirus glycoprotein B is required for virus entry and cell-to-cell spread but not for virion attachment, assembly, or egress. J Virol. 83(8): 3891-903.
CMV CMV Glycoprotein B Proteins
- Human cytomegalovirus (HCMV) Glycoprotein B / gB Protein (His Tag)10202-V08H1
- Human cytomegalovirus (HCMV) Glycoprotein B / gB Protein (Fc Tag)10202-V02H1
- Human cytomegalovirus (HCMV) Glycoprotein B / gB Protein10202-VCCH1
- BiTE Antikörper-Konstrukte zur Therapie von Influenza A-und Cytomegalovirus-InfektionenAuthorPendzialek, DBJ;Year2016JournalThesisFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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