Sino Biological/QPCT Protein, Human, Recombinant (His Tag)/1/13752-H07B
商品编号：
13752-H07B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
QPCT Protein, Human, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human QPCT (Q16769-1) (Ala33-Leu361) was fused with a polyhistide tag at the N-terminus.
Accession#
Q16769-1
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
His
Molecule Mass
The recombinant human QPCT consists of 345 amino acids and has a calculated molecular mass of 39.7 kDa. The recombinant protein migrates as an approximately 38 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glyPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
QPCT Protein, Human, Recombinant (His Tag): Images
QPCT Protein, Human, Recombinant (His Tag): Synonyms
GCT Protein, Human; QC Protein, Human; sQC Protein, Human
QPCT Background Information
Glutaminyl cyclase, also known as QPCT, can promote the N-terminal cyclization reaction of N-terminal pyroglutamate(pGlu). The pGlu formation from its glutaminyl precursor is required in the maturation of numerous bioactive peptides, while the aberrant formation of pGlu may be related to several pathological processes, such as osteoporosis and amyloidotic diseases. Glutaminyl cyclase"s structure reveals an alpha/beta scaffold akin to that of two-zinc exopeptidases but with several insertions and deletions, particularly in the active-site region. Glutaminyl cyclase"s amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase. It is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides.
Full Name
glutaminyl-peptide cyclotransferase
References
- Busby WH, et al. (1987) An enzyme(s) that converts glutaminyl-peptides into pyroglutamyl-peptides. Presence in pituitary, brain, adrenal medulla, and lymphocytes. J Biol Chem. 262(18):8532-6.
- Bateman RC, et al. (2001) Evidence for essential histidines in human pituitary glutaminyl cyclase. Biochemistry. 40(37):11246-50.
- Schilling S, et al. (2002) Heterologous expression and characterization of human glutaminyl cyclase: evidence for a disulfide bond with importance for catalytic activity. Biochemistry. 41 (35):10849-57.
- The importance of pyroglutamate in cellulase Cel7AAuthorDana, CM;Dotson-Fagerstrom, A;Roche, CM;Kal, SM;Chokhawala, HA;Blanch, HW;Clark, DS;Year2014JournalBiotechnol. Bioeng.ApplicationGlutaminyl Cyclase TreatmentPubMed ID: 24375151FoldedExpand
- Mutagenesis of Trichoderma reesei endoglucanase I: impact of expression host on activity and stability at elevated temperaturesAuthorChokhawala, HA;Roche, CM;Kim, TW;Year2015JournalBMC BiotechnologyApplicationenzymePubMed ID: 25879765FoldedExpand
- Engineering ionic liquid-tolerant cellulases for biofuels productionAuthorWolski, PW;Dana, CM;Clark, DS;Blanch, HW;Year2016JournalProtein Eng. Des. Sel.ApplicationenzymePubMed ID: 26819239FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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