Sino Biological/CD166/ALCAM Protein, Human, Recombinant (ECD, His Tag)/1/10045-H08H
商品编号：
10045-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
CD166/ALCAM Protein, Human, Recombinant (ECD, His Tag): Product Information
Purity
> 98 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Immobilized human ALCAM-His at 10 μg/ml (100 μl/well) can bind mouse CD6-Fc (Cat:50711-M02H), The EC50 of mouse CD6-Fc (Cat:50711-M02H) is 0.1-0.22 μg/ml.
Protein Construction
A DNA sequence encoding the extracellular domain (Met1-Ala526) of human ALCAM (NP_001618.2) was expressed with the C-terminal polyhistidine tag.
Accession#
NP_001618.2
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Trp 28
Molecule Mass
The recombinant human ALCAM consists of 510 amino acids and has a calculated molecular mass of 57.4 kDa. As a result of glycosylation, human ALCAM migrates as an approximately 78.3 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD166/ALCAM Protein, Human, Recombinant (ECD, His Tag): Images
Immobilized human ALCAM-His at 10 μg/ml (100 μl/well) can bind mouse CD6-Fc (Cat: 50711-M02H), The EC50 of mouse CD6-Fc (Cat: 50711-M02H) is 0.1-0.22 μg/ml.
CD166/ALCAM Protein, Human, Recombinant (ECD, His Tag): Synonyms
CD166 Protein, Human; MEMD Protein, Human
CD166/ALCAM Background Information
Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.
Full Name
activated leukocyte cell adhesion molecule
References
- Swart GW. (2002) Activated leukocyte cell adhesion molecule (CD166/ALCAM): developmental and mechanistic aspects of cell clustering and cell migration. Eur J Cell Biol. 81(6): 313-21.
- Fujiwara H, et al. (2003) Human blastocysts and endometrial epithelial cells express activated leukocyte cell adhesion molecule (ALCAM/CD166). J Clin Endocrinol Metab. 88(7): 3437-43.
- Jezierska A, et al. (2006) ALCAM/CD166 protects breast cancer cells against apoptosis and autophagy. Med Sci Monit. 12(8): BR263-73.
- Kahlert C, et al. (2009) Increased expression of ALCAM/CD166 in pancreatic cancer is an independent prognostic marker for poor survival and early tumour relapse. Br J Cancer. 101(3): 457-64.
Human CD166/ALCAM Proteins
- CD166/ALCAM Protein, Human, Recombinant (ECD, hFc Tag)10045-H02H
Mouse CD166/ALCAM Proteins
- CD166/ALCAM Protein, Mouse, Recombinant (His & hFc Tag)50005-M03H
- CD166/ALCAM Protein, Mouse, Recombinant (His Tag)50005-M08H
Rat CD166/ALCAM Proteins
- CD166/ALCAM Protein, Rat, Recombinant (His Tag)80221-R08H
- CD166/ALCAM Protein, Rat, Recombinant (hFc Tag)80221-R02H
Cynomolgus CD166/ALCAM Proteins
- CD166/ALCAM Protein, Cynomolgus, Recombinant (His Tag)90227-C08H
- CD166/ALCAM Protein, Cynomolgus, Recombinant (hFc Tag)90227-C02H
- Glycosylation-dependent binding of galectin-8 to activated leukocyte cell adhesion molecule (ALCAM/CD166) promotes its surface segregation on breast cancer cellsAuthorFernández, MM;Ferragut, F;Cárdenas Delgado, VM;Bracalente, C;Bravo, AI;Cagnoni, AJ;Nuñez, M;Morosi, LG;Quinta, HR;Espelt, MV;Troncoso, MF;Wolfenstein-Todel, C;Mariño, KV;Malchiodi, EL;Rabinovich, GA;Elola, MT;Year2016JournalBiochim. Biophys. ActaApplicationSPRFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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