Sino Biological/IFNAR2蛋白，人，重组（hFc标签）/1/10359-H02H
商品编号：
10359-H02H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IFNAR2 Protein, Human, Recombinant (hFc Tag): Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Measured by its ability to inhibit rh IFNβ mediated protection of WISH Human amnion cells infected with vesicular stomatitis virus (VSV) to viral lysis. The EC50 for this effect is typically 0.2-1.2 μg/mL.2. Measured by its binding ability in a functional ELISA. Immobilized PPICZa-IFNA2 (Cat:13833-HNAY) at 10 μg/mL (100 μL/well) can bind IFNaR2-Fc (Cat:10359-H02H), the EC50 of IFNaR2-Fc (Cat:10359-H02H) is 60-200 ng/mL.
Protein Construction
A DNA sequence encoding the extracellular domain of human IFNAR2 isoform a (NP_997468.1) (Met 1-Lys 243) was fused with the Fc region of human IgG1 at the C-terminus.
Accession#
NP_997468.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ile 27
Molecule Mass
The recombinant human IFNAR2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 458 amino acids and predicts a molecular mass of 51.8 kDa. As a result of glycosylation, the apparent molecular mass of rh IFNAR2/Fc monomer is approximately 65-75 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IFNAR2 Protein, Human, Recombinant (hFc Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized PPICZa-IFNA2 (Cat:13833-HNAY) at 10 μg/mL (100 μL/well) can bind IFNaR2-Fc (Cat:10359-H02H), the EC50 of IFNaR2-Fc (Cat:10359-H02H) is 60-200 ng/mL.
IFNAR2 Protein, Human, Recombinant (hFc Tag): Synonyms
IFN-alpha-REC Protein, Human; IFN-R Protein, Human; IFNABR Protein, Human; IFNARB Protein, Human
IFNAR2 Background Information
Interferon-alpha/beta receptor beta chain (IFNAR2) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulate Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Initial cell-surface IFNAR2 expression at diagnosis assessed by flow cytometry was widely distributed but showed overall significantly higher expression in CML patients when compared with normal controls. In 15 fresh patients who subsequently received IFNα therapy, IFNAR2 expression at diagnosis was significantly higher in cytogenetic good responders than in poor responders. Down-regulation of IFNAR2 expression during IFNα therapy was observed only in good responders but not in poor responders. The encoded protein also functions as an antiviral factor. IFNAR2 may associate with IFNAR1 to form the type I interferon receptor. This protein serves as a receptor for interferons alpha and beta. IFNAR2 is also involved in IFN-mediated STAT1, STAT2, and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity. Following binding of IFNα2, IFNAR2 is internalized, but, instead of being routed towards degradation as it is when complexed to IFNβ, it recycles back to the cell surface.
Full Name
interferon (alpha, beta and omega) receptor 2
References
- Ito K, et al. (2004) Initial expression of interferon alpha receptor 2 (IFNAR2) on CD34-positive cells and its down-regulation correlate with clinical response to interferon therapy in chronic myelogenous leukemia. Eur J Haematol. 73(3): 191-205.
- Kim SH, et al. (1997) Mammalian type I interferon receptors consists of two subunits: IFNaR1 and IFNaR2. Gene. 196(1-2): 279-86.
- Saleh AZ, et al. (2004) Regulated proteolysis of the IFNaR2 subunit of the interferon-alpha receptor. Oncogene. 23(42): 7076-86.
Human IFNAR2 Proteins
- IFNAR2 Protein, Human, Recombinant (His Tag)10359-H08H
- IFNAR2 Protein, Human, Recombinant (His Tag), Biotinylated10359-H08H-B
Cynomolgus IFNAR2 Proteins
- IFNAR2 Protein, Cynomolgus, Recombinant (ECD, His Tag)90112-C08H
- Microfluidic Platform for Assessment of Therapeutic Proteins Using Molecular Charge Modulation Enhanced Electrokinetic Concentration AssaysAuthorOuyang, W;Ko, SH;Wu, D;Wang, AY;Barone, PW;Hancock, WS;Han, J;Year2016JournalAnal. Chem.Applicationassay validationPubMed ID: 27624735FoldedExpand
- Microfluidic platform for rapid biologics activity assessment using molecular charge modulation and electrokinetic concentration based receptor assaysAuthorOuyang, W;Year2016JournalThesisApplicationbindingFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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