Sino Biological/IRE1 Protein, Human, Recombinant (aa 465-977, His & GST Tag)/1/11905-H20B
商品编号：
11905-H20B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IRE1 Protein, Human, Recombinant (aa 465-977, His & GST Tag): Product Information
Purity
> 80 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
The specific activity was determined to be 5 nmol/min/mg using MBP as substrate
Protein Construction
A DNA sequence encoding the human ERN1 isoform 1 (O75460-1) cytoplasmic domain (Pro 465-Leu 977) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Accession#
O75460-1
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human ERN1/GST chimera consists of 750 amino acids and has a calculated molecular mass of 86 kDa. It migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IRE1 Protein, Human, Recombinant (aa 465-977, His & GST Tag): Images
IRE1 Protein, Human, Recombinant (aa 465-977, His & GST Tag): Synonyms
hIRE1p Protein, Human; IRE1 Protein, Human; IRE1a Protein, Human; IRE1P Protein, Human
IRE1 Background Information
Endoplasmic reticulum stress and hypoxia are necessary components of malignant tumors growth and suppression of ERN1 (from endoplasmic reticulum to nuclei-1) signalling pathway, which is linked to the apoptosis and cell death processes, significantly decreases proliferative processes. An enhanced expression of TP53 gene in ERN1 knockdown glioma cells correlates with the decreased level of ubiquitin ligase MDM2 and increased expression level of USP7 which deubiquitinates TP53 and MDM2 and induces TP53-dependent cell growth repression and apoptosis. Thus, the expression of genes encoding TP53 and related to TP53 factors depends upon the endoplasmic reticulum stress signaling as well as on hypoxia, and correlates with suppression of glioma growth under ERN1 knockdown. The dependence of insulin-like growth binding proteins as well as IGF2BP3 and HTRA1 gene expressions in U87 glioma cells on ERN1 signaling enzyme function and hypoxia, indicating its participation in the regulation of metabolic and proliferative processes via IGF/INS receptors, because endoplasmic reticulum stress is an important component of tumor growth and metabolic diseases.
Full Name
endoplasmic reticulum to nucleus signaling 1
References
- Trentmann,S.M.etal., 2000, Plant Mol Biol. 44(1):11-25.
- IwawakiT., et al., 2001, Nat. Cell Biol. 3:158-64.
- LiuC.Y., et al., 2003, J. Biol. Chem. 278:17680-7.
- OppermannF.S., et al., 2009, Mol. Cell. Proteomics 8:1751-64.
Human IRE1 Proteins
- IRE1 Protein, Human, Recombinant (aa 465-977)11905-HNCB
- A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcBAuthorY Lu, FX Liang, X WangYear2014JournalMolecular cellApplicationbindingPubMed ID: 25087875FoldedExpand
- Ubiquitination of inositol-requiring enzyme 1 (IRE1) by the E3 ligase CHIP mediates the IRE1/TRAF2/JNK pathwayAuthorX Zhu, J Zhang, H Sun, C Jiang, Y Dong, Q Shan…Year2014JournalJOURNAL OF BIOLOGICAL CHEMISTRYApplicationsubstratePubMed ID: 25225294FoldedExpand
- miR-216b regulation of c-Jun mediates GADD153/CHOP-dependent apoptosisAuthorXu, Z;Bu, Y;Chitnis, N;Koumenis, C;Fuchs, SY;Diehl, JA;Year2016JournalNat CommunFoldedExpand
- Peptidomimetic-based identification of FDA approved compounds inhibiting IRE1 activityAuthorDoultsinos, D;Carlesso, A;Chintha, C;Paton, JC;Paton, AW;Samali, A;Chevet, E;Eriksson, LA;Year2020JournalFEBS J.ApplicationMSTPubMed ID: 32446294FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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