Sino Biological/TNFRSF1A Protein, Human, Recombinant (His Tag)/1/10872-H08H
商品编号：
10872-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TNFRSF1A Protein, Human, Recombinant (His Tag): Product Information
Purity
> 92 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Measured by its binding ability in a functional ELISA.Immobilized human TNFRI-His at 10 μg/mL (100 μl/well) can bind biotinylated human TNFα. The EC50 of biotinylated human TNFα is 5-12 μg/mL.2. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-1μg/mL in the presence of 0.25 ng/mL recombinant human TNFα.
Protein Construction
A DNA sequence encoding the human TNFRI (NP_001056.1) extracellular domain (Met 1-Thr 211) was fused with a polyhistidine tag at the C-terminus.
Accession#
NP_001056.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ile 22
Molecule Mass
The recombinant human TNFRI consists of 201 amino acids after removal of the signal peptide and has a predicted molecular mass of 22.7 kDa. As a result of glycosylation, the apparent molecular mass of rh TNFRI is 30-35 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
TNFRSF1A Protein, Human, Recombinant (His Tag): Images
Measured by its ability to inhibit TNFα-mediated cytotoxicity in L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-1μg/mL in the presence of 0.25 ng/mL recombinant human TNFα.
TNFRSF1A Protein, Human, Recombinant (His Tag): Synonyms
CD120a Protein, Human; FPF Protein, Human; MS5 Protein, Human; p55 Protein, Human; p55-R Protein, Human; p60 Protein, Human; TBP1 Protein, Human; TNF-R Protein, Human; TNF-R-I Protein, Human; TNF-R55 Protein, Human; TNFAR Protein, Human; TNFR1 Protein, Human; TNFR1-d2 Protein, Human; TNFR55 Protein, Human; TNFR60 Protein, Human
TNFRSF1A Background Information
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.
Full Name
tumor necrosis factor receptor superfamily member 1A
Research Areas
- Neuroinflammation
Related Pathways
Death Receptor Signaling
NF-kB (NFkB) Pathway
TNF Signaling
References
- Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
- Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
- Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
- Cottin V, et al. (2002) Restricted localization of the TNF receptor CD120a to lipid rafts: a novel role for the death domain. The journal of immunology. 168: 4095-102.
Human TNFRSF1A Proteins
- TNFRSF1A Protein, Human, Recombinant (His & hFc Tag)10872-H03H
- TNFRSF1A Protein, Human, Recombinant (ECD, hFc Tag)10872-H02H
Mouse TNFRSF1A Proteins
- TNFRSF1A Protein, Mouse, Recombinant (His Tag)50496-M08H
- TNFRSF1A Protein, Mouse, Recombinant (hFc Tag)50496-M02H
Rat TNFRSF1A Proteins
- TNFRSF1A Protein, Rat, Recombinant (His Tag)80181-R08H
- TNFRSF1A Protein, Rat, Recombinant (hFc Tag)80181-R02H
- Novel human three-domain antibody fragments against sTNFα as well as tmTNFα with high affinity generated by the combination of ribosome display and E.coli expression systemAuthorZhao, XL;Tian, LF;Zhang, SJ;Li, JM;Feng, H;Wang, LM;Wang, S;Wang, J;Wang, T;Chen, WQ;Year2016JournalScand. J. Immunol.ApplicationbindingFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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