Sino Biological/TNFR2/TNFRSF1B Protein, Human, Recombinant (aa 1-268, 196 Met/Arg, His Tag)/1/10417-H08H1
商品编号：
10417-H08H1
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TNFR2/TNFRSF1B Protein, Human, Recombinant (aa 1-268, 196 Met/Arg, His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 5-40 ng/mL in the presence of 1 ng/mL recombinant human TNF-α.
Protein Construction
A DNA sequence encoding the human TNFRSF1B (EAW71731.1) (Met1-Arg268, natural variant 196 Met/Arg) was expressed with a C-terminal polyhistidine tag.
Accession#
EAW71731.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Leu 23
Molecule Mass
The recombinant human TNFRSF1B comprises 257 amino acids and has a predicted molecular mass of 27.7 kDa. The apparent molecular mass of the protein is approximately 43 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
TNFR2/TNFRSF1B Protein, Human, Recombinant (aa 1-268, 196 Met/Arg, His Tag): Images
Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 5-40 ng/mL in the presence of 1 ng/mL recombinant human TNF-α.
TNFR2/TNFRSF1B Protein, Human, Recombinant (aa 1-268, 196 Met/Arg, His Tag): Synonyms
CD120b Protein, Human; p75 Protein, Human; p75TNFR Protein, Human; TBPII Protein, Human; TNF-R-II Protein, Human; TNF-R75 Protein, Human; TNFBR Protein, Human; TNFR1B Protein, Human; TNFR2 Protein, Human; TNFR80 Protein, Human
TNFR2/TNFRSF1B Background Information
Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
tumor necrosis factor receptor superfamily, member 1b
Research Areas
- Neuroinflammation
References
- Komata T, et al. (1999) Association of tumor necrosis factor receptor 2 (TNFR2) polymorphism with susceptibility to systemic lupus erythematosus. Tissue Antigens. 53(6): 527-33.
- Tsuchiya N, et al. (2001) Analysis of the association of HLA-DRB1, TNFalpha promoter and TNFR2 (TNFRSF1B) polymorphisms with SLE using transmission disequilibrium test. Genes Immun. 2(6): 317-22.
- Guo G, et al. (1999) Role of TNFR1 and TNFR2 receptors in tubulointerstitial fibrosis of obstructive nephropathy. Am J Physiol. 277(5): 766-72.
Human TNFR2/TNFRSF1B Proteins
- TNFR2/TNFRSF1B Protein, Human, Recombinant (ECD, His Tag), Biotinylated10417-H08H-B
- TNFR2/TNFRSF1B Protein, Human, Recombinant (ECD, His Tag)10417-H08H
- TNFR2/TNFRSF1B Protein, Human, Recombinant (ECD, His & hFc Tag)10417-H03H
Mouse TNFR2/TNFRSF1B Proteins
- TNFR2/TNFRSF1B Protein, Mouse, Recombinant (hFc Tag)50128-M02H
- TNFR2/TNFRSF1B Protein, Mouse, Recombinant (His Tag)50128-M08H
Cynomolgus, Rhesus TNFR2/TNFRSF1B Proteins
- TNFR2/TNFRSF1B Protein, Cynomolgus, Rhesus, Recombinant (hFc Tag)90102-C02H
- TNFR2/TNFRSF1B Protein, Cynomolgus, Rhesus, Recombinant (His Tag)90102-C08H
- Novel human three-domain antibody fragments against sTNFα as well as tmTNFα with high affinity generated by the combination of ribosome display and E.coli expression systemAuthorZhao, XL;Tian, LF;Zhang, SJ;Li, JM;Feng, H;Wang, LM;Wang, S;Wang, J;Wang, T;Chen, WQ;Year2016JournalScand. J. Immunol.FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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