Sino Biological/CD16/FCGR3 Protein, Rhesus, Recombinant (His Tag)/1/90013-C08H
商品编号：
90013-C08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
CD16/FCGR3 Protein, Rhesus, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA.Immobilized Rhesus FCGR3-His (cat:90013-C08H) at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc) (Cat:10702-HNAC). The EC50 of human IgG1 (Fc) (Cat:10702-HNAC) is 0.35-0.81 μg/ml.
Protein Construction
A DNA sequence encoding the rhesus CD16 (NP_001258584.1) extracellular domain (Met 1-Gly 206) was fused with a polyhistidine tag at the C-terminus.
Accession#
NP_001258584.1
Expressed Host
HEK293 Cells
Species
Rhesus
Predicted N Terminal
Gly 17
Molecule Mass
The recombinant rhesus CD16 consists of 201 amino acids and has a calculated molecular mass of 22.7 kDa.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD16/FCGR3 Protein, Rhesus, Recombinant (His Tag): Images
CD16/FCGR3 Background Information
Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
Full Name
Fc receptor, IgG, low affinity III
Research Areas
- Fc Receptors
References
- Edberg JC, et al. (2002) Genetic linkage and association of Fcgamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.
- Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.
- Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.
Mouse CD16/FCGR3 Proteins
- CD16/FCGR3 Protein, Mouse, Recombinant (ECD, His Tag)50326-M08H
- CD16/FCGR3 Protein, Mouse, Recombinant (ECD, His & AVI Tag), Biotinylated50326-M27H-B
Cynomolgus CD16/FCGR3 Proteins
- CD16/FCGR3 Protein, Cynomolgus, Recombinant (His Tag)90013-C08H1
Rhesus CD16/FCGR3 Proteins
- CD16/FCGR3 Protein, Rhesus, Recombinant (His & AVI Tag)90013-C27H
- CD16/FCGR3 Protein, Rhesus, Recombinant (His & AVI Tag), Biotinylated90013-C27H-B
- Detection of drug specific circulating immune complexes from in vivo cynomolgus monkey serum samples ☆AuthorPierog, P;Krishna, M;Yamniuk, A;Chauhan, A;Year2014JournalJournal of Immunological MethodsApplicationbindingPubMed ID: 25462536FoldedExpand
- Detection of drug specific circulating immune complexes from in vivo cynomolgus monkey serum samplesAuthorPierog, P;Krishna, M;Yamniuk, A;Chauhan, A;DeSilva, B;Year2015JournalJ. Immunol. MethodsApplicationbindingPubMed ID: 25462536FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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