Sino Biological/IL12B Protein, Human, Recombinant (His Tag)/1/10052-H08H
商品编号：
10052-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IL12B Protein, Human, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA.
Protein Construction
A DNA sequence encoding the p40 subunit of human IL12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus.
Accession#
NP_002178.2
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ile 23
Molecule Mass
The recombinant human IL12B consists of 317 amino acids and has a predicted molecular mass of 36.2 kDa. As a result of glycosylation, the apparent molecular mass of rhIL12B is approximately 40-50 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL12B Protein, Human, Recombinant (His Tag): Images
IL12B Protein, Human, Recombinant (His Tag): Synonyms
CLMF Protein, Human; CLMF2 Protein, Human; IL-12B Protein, Human; IL12 p40 Protein, Human; IMD28 Protein, Human; IMD29 Protein, Human; NKSF Protein, Human; NKSF2 Protein, Human
IL12B Background Information
Subunit beta of interleukin 12 (also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) (IL12B) is a subunit of human interleukin 12. IL12B/IL-12B is a cytokine that acts on T and natural killer cells and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor-like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. IL12B/IL-12B is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine is important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. IL12B/IL-12B associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine that functions in innate and adaptive immunity.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
interleukin 12B
Related Pathways
IL12 signaling pathway
References
- Taoufik Y, et al. (1997) Human immunodeficiency virus gp120 inhibits interleukin-12 secretion by human monocytes: an indirect interleukin-10-mediated effect. Blood. 89 (8): 2842-8.
- Fantuzzi L, et al. (1996) Induction of interleukin-12 (IL-12) by recombinant glycoprotein gp120 of human immunodeficiency virus type 1 in human monocytes/macrophages: requirement of gamma interferon for IL-12 secretion. J Virol. 70 (6): 4121-4.
- Aragane Y, et al. (1995) IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 153 (12): 5366-72.
Human IL12B Proteins
- IL12B Protein, Human, Recombinant (hFc Tag)10052-H02H
Mouse IL12B Proteins
- IL12B Protein, Mouse, Recombinant (hFc Tag)51004-M02H
- IL12B Protein, Mouse, Recombinant (His Tag)51004-M08H
- IL12B Protein, Mouse, Recombinant51004-MCCH
Rat IL12B Proteins
- IL12B Protein, Rat, Recombinant (hFc Tag)80352-R02H
- IL12B Protein, Rat, Recombinant (His Tag)80352-R08H
Marmoset IL12B Proteins
- IL12B Protein, Marmoset, Recombinant (hFc Tag)90289-C02H
Rabbit IL12B Proteins
- IL12B Protein, Rabbit, Recombinant (His Tag)65008-T08H
- Host ICAMs play a role in cell invasion by Mycobacterium tuberculosis and Plasmodium falciparumAuthorBhalla, K;Chugh, M;Mehrotra, S;Rathore, S;Tousif, S;Prakash Dwivedi, V;Prakash, P;Kumar Samuchiwal, S;Kumar, S;Kumar Singh, D;Ghanwat, S;Kumar, D;Das, G;Mohmmed, A;Malhotra, P;Ranganathan, A;Year2015JournalNat CommunApplicationELISAPubMed ID: 25586702FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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