Sino Biological/Anti-TNF-alpha Antibody, Mouse Monoclonal/1/10602-MM01
商品编号：
10602-MM01
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Anti-TNF-alpha Antibody, Mouse Monoclonal General Information
Product name
Anti-TNF-alpha Antibody, Mouse Monoclonal
Validated applications
ELISA,ELISA(Cap),FCM
Species reactivity
Reacts with: Human
Specificity
Human TNF-alpha
Immunogen
Recombinant Human TNFα protein (Catalog#10602-HNAE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Tumor necrosis factor-alpha (rhTNFα; Catalog#10602-HNAE; Val 77-Leu 233; NP_000585.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #9A7A5G9
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-TNF-alpha Antibody, Mouse Monoclonal Validated Applications
| Application | Dilution |
|---|---|
| ELISA | 1:1000-1:2000 |
| FCM | 1:25-1:100 |
| ELISA(Cap) | 1:250-1:2000 |
Notes
| ELISA(Cap)： | This antibody will detect Human TNF-alpha / TNFA / TNFSF2 in ELISA pair set (Catalog: # SEKA10602). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 10602-MM08). |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Anti-TNF-alpha Antibody, Mouse Monoclonal Images
Flow cytometric analysis of Purified anti-Human TNFa on CHO transfected cells and stimulated lymphocytes. Left panel The transfected cells stained with Purified Mouse anti-TNFa (Bold line hisgram), To demonstrate specificity of staining, the binding of 10602-MM01 was blocked by the preincubation of the purified antibody with molar excess of recombinant human TNFa (5 μg, Cat. No. 10602-HNAE) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody. Right panel Human PBMC were stimulated for 4 hrs with Leukocyte Activation Cocktail (with BD GolgiPlug™, BD Pharmingen™ Cat. No. 550583), then cells were harvested and treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714).
Anti-TNF-alpha Antibody, Mouse Monoclonal: Synonyms
Anti-DIF Antibody; Anti-TNF-alpha Antibody; Anti-TNFA Antibody; Anti-TNFSF2 Antibody
TNF-alpha Background Information
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & Antibody
TNF-alpha Protein & Antibody- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
tumor necrosis factor
Research Areas
- Cancer Drug Targets
- Neuroinflammation
Related Pathways
Death Receptor Signaling
NF-kB (NFkB) Pathway
IL17 signaling pathway
References
- Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
- Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
- Approaching near real-time biosensing: microfluidic microsphere based biosensor for real-time analyte detectionAuthorCohen, N;Sabhachandani, P;Golberg, A;Konry, T;Year2015JournalBiosens BioelectronPubMed ID: 25497985FoldedExpand
- Microsphere-based immunoassay integrated with a microfluidic network to perform logic operationsAuthorSabhachandani, P;Cohen, N;Sarkar, S;Konry, T;Year2015JournalMicrochimica ActaApplicationanti-TNF-α antibody detection with microsphere sensorsFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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