Sino Biological/Fractalkine Protein, Human, Recombinant (His Tag)/1/10636-H08H
商品编号：
10636-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Fractalkine Protein, Human, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its ability to compete with biotinylated recombinant human CX3CL1-His for binding to immobilized recombinant mouse CD4-his (Cat:50134-M08H）in a functional ELISA.
Protein Construction
A DNA sequence encoding the human CX3CL1 (NP_002987.1) extracellular domain (Met 1-Arg 339) with a C-terminal polyhistidine tag was expressed.
Accession#
NP_002987.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Gln 25
Molecule Mass
The secreted recombinant human CX3CL1 comprises 329 amino acids with a predicted molecular mass of 35 kDa. As a result of different glycosylation, rhCX3CL1 migrates between 50-70 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Fractalkine Protein, Human, Recombinant (His Tag): Images
Fractalkine Protein, Human, Recombinant (His Tag): Synonyms
ABCD-3 Protein, Human; C3Xkine Protein, Human; CXC3 Protein, Human; CXC3C Protein, Human; fractalkine Protein, Human; neurotactin Protein, Human; NTN Protein, Human; NTT Protein, Human; SCYD1 Protein, Human
Fractalkine Background Information
Fractalkine or Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a member of the CX3C chemokine family. Fractalkine / CX3CL1 is a unique chemokine that functions not only as a chemoattractant but also as an adhesion molecule and is expressed on endothelial cells activated by proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Fractalkine/CX3CL1 is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Fractalkine / CX3CL1 is associated with dendritic cells (DC) in epidermis and lymphoid organs. The fractalkine receptor, CX3CR1, is expressed on cytotoxic effector lymphocytes, including natural killer (NK) cells and cytotoxic T lymphocytes, which contain high levels of intracellular perforin and granzyme B, and on macrophages. Soluble fractalkine causes migration of NK cells, cytotoxic T lymphocytes, and macrophages, whereas the membrane-bound form captures and enhances the subsequent migration of these cells in response to secondary stimulation with other chemokines.
Full Name
chemokine (C-X3-C motif) ligand 1
Research Areas
- Neuroinflammation
References
- Imai T, et al. (1997) Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell. 91(4): 521-30.
- Papadopoulos EJ, et al. (1999) Fractalkine, a CX3C chemokine, is expressed by dendritic cells and is up-regulated upon dendritic cell maturation. Eur J Immunol. 29 (8): 2551-9.
- Umehara H, et al. (2004) Fractalkine in vascular biology: from basic research to clinical disease". Arterioscler. Thromb Vasc Biol. 24 (1): 34-40.
Mouse Fractalkine Proteins
- Fractalkine Protein, Mouse, Recombinant (His Tag)50917-M08Y
- Fractalkine Protein, Mouse, Recombinant (His Tag)50917-M08Y2
Cynomolgus, Rhesus Fractalkine Proteins
- Fractalkine Protein, Cynomolgus, Rhesus, Recombinant (His Tag)90316-C08H
- Fractalkine Protein, Cynomolgus, Rhesus, Recombinant (hFc Tag)90316-C02H
Canine Fractalkine Proteins
- Fractalkine Protein, Canine, Recombinant (hFc Tag)70071-D02H
- Fractalkine Protein, Canine, Recombinant (His Tag)70071-D08H
- Fractalkine Protein, Canine, Recombinant70071-DCCH
- P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathyAuthorWang, H;Hong, LJ;Huang, JY;Jiang, Q;Tao, RR;Tan, C;Lu, NN;Wang, CK;Ahmed, MM;Lu, YM;Liu, ZR;Shi, WX;Lai, EY;Wilcox, CS;Han, F;Year2015JournalCell ResearchApplicationcell-basedFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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