Sino Biological/RBP4 Protein, Human, Recombinant (His Tag)/1/10354-H08H
商品编号：
10354-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
RBP4 Protein, Human, Recombinant (His Tag): Product Information
Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4.The 50% binding concentration (BC50) is > 1.0 µM.
Protein Construction
A DNA sequence encoding the pro form of human RBP4 (NP_006735.2) (Met 1-Leu 201) was expressed with a C-terminal polyhistidine tag.
Accession#
NP_006735.2
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Glu 19
Molecule Mass
The recombinant human RBP4 consists of 194 amino acids after removal of the signal peptide and migrates as an approximately 23 kDa protein as predicted.
Formulation
Lyophilized from sterile PBS, pH 7.2Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
RBP4 Protein, Human, Recombinant (His Tag): Images
RBP4 Protein, Human, Recombinant (His Tag): Synonyms
RDCCAS Protein, Human
RBP4 Background Information
Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM).
Full Name
retinol binding protein 4, plasma
References
- Yang Q, et al. (2005) Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature. 436(7049): 356-62.
- Choi SH, et al. (2008) High plasma retinol binding protein-4 and low plasma adiponectin concentrations are associated with severity of glucose intolerance in women with previous gestational diabetes mellitus. J Clin Endocrinol Metab. 93(8): 3142-8.
- Tepper BJ, et al. (2010) Serum retinol-binding protein 4 (RBP4) and retinol in a cohort of borderline obese women with and without gestational diabetes. Clin Biochem. 43(3): 320-3.
Mouse RBP4 Proteins
- RBP4 Protein, Mouse, Recombinant (His Tag)50170-M08H
Rat RBP4 Proteins
- RBP4 Protein, Rat, Recombinant (His Tag)80008-R08H
Cynomolgus RBP4 Proteins
- RBP4 Protein, Cynomolgus, Recombinant (His Tag)90220-C08H
Canine RBP4 Proteins
- RBP4 Protein, Canine, Recombinant (hFc Tag)70006-D02H
- RBP4 Protein, Canine, Recombinant (His Tag)70006-D08H
- Retinol binding protein 4 stimulates hepatic sterol regulatory element-binding protein 1 and increases lipogenesis through the peroxisome proliferator-activated receptor-γ coactivator 1β-dependent pathwayAuthorXia, M;Liu, Y;Guo, H;Wang, D;Wang, Y;Ling, W;Year2013JournalHepatologyApplicationcell-based (induction)PubMed ID: 23300015FoldedExpand
- Involvement of RBP4 in hyperinsulinism-induced vascular smooth muscle cell proliferationAuthorF Li, K Xia, MSA Sheikh, J Cheng, C Li, T YangYear2014JournalEndocrinePubMed ID: 24888764FoldedExpand
- Retinol binding protein 4 promotes hyperinsulinism‑induced proliferation of rat aortic smooth muscle cellsAuthorLi, F;Xia, K;Sheikh, MS;Cheng, J;Li, C;Yang, T;Year2014JournalMol Med RepApplicationcell-basedPubMed ID: 24604418FoldedExpand
- Retinol binding protein 4 induces mitochondrial dysfunction and vascular oxidative damageAuthorWang, J;Chen, H;Liu, Y;Zhou, W;Sun, R;Xia, M;Year2015JournalAtherosclerosisPubMed ID:25875385FoldedExpand
- Comparative pharmacokinetics and pharmacodynamics of the advanced Retinol-Binding Protein 4 antagonist in dog and cynomolgus monkeyAuthorRacz, B;Varadi, A;Pearson, PG;Petrukhin, K;Year2020JournalPLoS ONEPubMed ID: 31978148FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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