Sino Biological/IL-1α/IL1A蛋白，小鼠，重组/1/50114-MNAE
商品编号：
50114-MNAE
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IL-1 alpha/IL1A Protein, Mouse, Recombinant: Product Information
Purity
> 92 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
1. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 2-10 pg/mL.2. Measured by its binding ability in a functional ELISA.3. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The EC50 of mouse IL1R1 is 0.13 μg/mL.4. Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells.The ED50 for this effect is typically 3-15 ng/mL.
Protein Construction
A DNA sequence encoding the mature form of mouse IL1A (NP_034684.2) (Ser 115-Ser 270) was expressed, with an initial Met at the C-terminus.
Accession#
NP_034684.2
Expressed Host
E. coli
Species
Mouse
Predicted N Terminal
Met
Molecule Mass
The recombinant mouse IL1A consists of 157 amino acids and migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions as predicted.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
IL-1 alpha/IL1A Protein, Mouse, Recombinant: Images
Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells.The ED50 for this effect is typically 3-15 ng/mL.
IL-1 alpha/IL1A Protein, Mouse, Recombinant: Synonyms
Il-1a Protein, Mouse
IL-1 alpha/IL1A Background Information
IL-1 alpha is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. Cytokines can be classified into two groups: pro- and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6, and TNF-alpha, are predominantly derived from the innate immune cells and Th1 cells. Anti-inflammatory cytokines, including IL-10, IL-4, IL-13, and IL-5, are synthesized from Th2 immune cells. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
interleukin 1, alpha
Research Areas
- Neuroinflammation
Related Pathways
IL1 signaling pathway
References
- Nicklin MJ,et al. (1994) A physical map of the region encompassing the human interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist genes. Genomics. 19(2):382-4.
- March CJ, et al. (1985) Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature. 315(6021):641-7.
- Bankers-Fulbright JL, et al. (1996) Interleukin-1 signal transduction. Life Sci. 59(2):61-83.
- Dinarello CA, et al. (1997) Induction of interleukin-1 and interleukin-1 receptor antagonist. Semin Oncol. 24 (3 Suppl 9):S9-81-S9-93.
Human IL-1 alpha/IL1A Proteins
- IL-1 alpha/IL1A Protein, Human, Recombinant10128-HNCE
- IL-1 alpha/IL1A Protein, Human, Recombinant10128-HNCH
Rat IL-1 alpha/IL1A Proteins
- IL-1 alpha/IL1A Protein, Rat, Recombinant80385-RNAE
- Downstream of Mutant KRAS, the Transcription Regulator YAP Is Essential for Neoplastic Progression to Pancreatic Ductal AdenocarcinomaAuthorZhang, W;Nandakumar, N;Shi, Y;Manzano, M;Smith, A;Graham, G;Gupta, S;Vietsch, EE;Laughlin, SZ;Wadhwa, M;Chetram, M;Joshi, M;Wang, F;Kallakury, B;Toretsky, J;Wellstein, A;Yi, C;Year2014JournalSci SignalApplicationcell-basedPubMed ID: 24803537FoldedExpand
- Axl deficiency promotes the neuroinvasion of Japanese encephalitis virus by enhancing IL-1α production from pyroptotic macrophagesAuthorWang, ZY;Zhen, ZD;Fan, DY;Qin, CF;Han, DS;Zhou, HN;Wang, PG;An, J;Year2020JournalJ. Virol.Applicationcell cilturePubMed ID: 32611752FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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