Sino Biological/GFP Protein, Aequorea victoria, Recombinant (His Tag)/1/13105-S07E
商品编号：
13105-S07E
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
GFP Protein, Aequorea victoria, Recombinant (His Tag): Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Activity Assay
Protein Construction
A DNA sequence encoding the Aequorea victoria GFP (AAB65663) (Ser2-Lys238) was expressed with a polyhistide tag at the N-terminus.
Accession#
AAB65663
Expressed Host
E. coli
Species
Aequorea victoria
Predicted N Terminal
Met
Molecule Mass
The recombinant Aequorea victoria GFP consisting of 253 amino acids and has a calculated molecular mass of 28.7 kDa. It migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions as predicted.
Formulation
Lyophilized from sterile PBS, pH7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
GFP Protein, Aequorea victoria, Recombinant (His Tag): Images
GFP Protein, Aequorea victoria, Recombinant (His Tag): Synonyms
GFP Protein, Aequorea victoria
GFP Background Information
The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.
References
- Evdokimov et al. (2006). EMBO Rep, 7 (10):1006–1012 / pmid: 16936637.
- Haas et al. (1996). Curr Biol, 6 (3): 315–324 / pmid: 8805248.
- Kremers et al. (2006). Biochemistry, 45 (21): 6570–6580 / pmid: 16716067.
- Li et al. (1998). J Biol Chem, 273 (52): 34970–34975 /pmid: 9857028.
- Reid and Flynn (1997). Biochemistry, 36 (22): 6786–6791 / pmid: 9184161.
- Shagin et al. (2004). Curr Biol, 21 (5): 841–850 / pmid: 14963095.
Aequorea victoria GFP Proteins
- GFP Protein, Aequorea victoria, Recombinant (His Tag)69001-S08E
- Nanosecond dynamics of calmodulin and ribosome-bound nascent chains studied by time-resolved fluorescence anisotropyAuthorLamprou, P;Kempe, D;Katranidis, A;Büldt, G;Fitter, J;Year2014JournalChembiochemPubMed ID: 24644265FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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