Sino Biological/PCSK9 Protein, Mouse, Recombinant (His Tag)/1/50251-M08H
商品编号：
50251-M08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PCSK9 Protein, Mouse, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.12 μg/ml.
Protein Construction
A DNA sequence encoding the full length of mouse PCSK9 (NP_705793.1) precursor (Met 1-Gln 694) was expressed, with a C-terminal polyhistidine tag.
Accession#
NP_705793.1
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Gln 35
Molecule Mass
The secreted recombinant mouse PCSK9 consists of 671 amino acids and has a calculated molecular mass of 72.6 kDa. As a result of proteolysis and glycosylation, the recombinant protein migrates as doublet with apparent molecular mass of approximately 19 & 65 kDa, cprresponding to the prodomain and the mature form of PCSK9 respectively in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 15mM Tris, 90mM NaCl, 50% Glycerol, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
PCSK9 Protein, Mouse, Recombinant (His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.12 μg/ml.
PCSK9 Protein, Mouse, Recombinant (His Tag): Synonyms
AI415265 Protein, Mouse; AI747682 Protein, Mouse; FH3 Protein, Mouse; HCHOLA3 Protein, Mouse; Narc1 Protein, Mouse; PC9 Protein, Mouse
PCSK9 Background Information
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
Full Name
proprotein convertase subtilisin/kexin type 9
Research Areas
- Serine Proteases and Regulators
References
- Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933.
- Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498
- Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73.
- Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875.
- Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
Human PCSK9 Proteins
- PCSK9 Protein, Human, Recombinant (His & AVI Tag), Biotinylated29698-H27H-B
- Human PCSK9 / NARC1 (D374Y, V474I, G670E) Protein (His Tag)10594-H08H1
- PCSK9 Protein, Human, Recombinant (mFc Tag)29698-H05H
- PCSK9 Protein, Human, Recombinant (His Tag), Biotinylated10594-H08H-B
- Human PCSK9 / NARC1 (D374Y, V474I, G670E) Protein (His Tag), Biotinylated10594-H08H1-B
- PCSK9 Protein, Human, Recombinant (His Tag)29698-H08H
- PCSK9 Protein, Human, Recombinant (D374Y, His Tag)29698-H08H1
- PCSK9 Protein, Human, Recombinant (D374Y, mFc Tag)29698-H05H1
- PCSK9 Protein, Human, Recombinant (D374Y, mFc Tag), Biotinylated29698-H05H1-B
- PCSK9 Protein, Human, Recombinant (His Tag), Biotinylated29698-H08H-B
Mouse PCSK9 Proteins
- PCSK9 Protein, Mouse, Recombinant (mFc Tag)50251-M05H
Rat PCSK9 Proteins
- PCSK9 Protein, Rat, Recombinant (His Tag)80005-R08H
Rhesus PCSK9 Proteins
- PCSK9 Protein, Rhesus, Recombinant (His Tag)11054-K08H
- PCSK9 Protein, Rhesus, Recombinant (mFc Tag)11054-K05H
- Low-density lipoprotein receptor represents an apolipoprotein E-independent pathway of Aβ uptake and degradation by astrocytesAuthorBasak, JM;Verghese, PB;Yoon, H;Kim, J;Holtzman, DM;Year2012JournalJ. Biol. Chem.PubMed ID: 22383525FoldedExpand
- Modulating Amyloid Beta Clearance by Altering Cellular and Whole Brain Apolipoprotein E MetabolismAuthorBasak, JM;Year2014JournalThesisFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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