Sino Biological/HER3/ERBB3蛋白，小鼠，重组（His-Tag）/1/51003-M08H
商品编号：
51003-M08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HER3/ERBB3 Protein, Mouse, Recombinant (His Tag): Product Information
Purity
> 93 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA.1. Immobilized mouse ErbB3-His (Cat: 51003-M08H) at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (isoform Beta1) (Cat: 11609-H01H2) with a linear range of 0.018-0.125 μg/ml.2. Immobilized mouse ErbB3-His (Cat: 51003-M08H) at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (aa 2-246)-Fc (Cat: 11609-H04) with a linear range of 6.25-100 ng/ml.
Protein Construction
A DNA sequence encoding the mouse ERBB3 (Q61526) extracellular domain (Met 1-His 641) was expressed, with a C-terminal polyhistidine tag.
Accession#
Q61526
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Ser 20
Molecule Mass
The secreted recombinant mouse ERBB3 comprises 633 amino acids and has a calculated molecular mass of 70 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 95 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
HER3/ERBB3 Protein, Mouse, Recombinant (His Tag): Images
Measured by its binding ability in a functional ELISA.1. Immobilized mouse ErbB3-His (Cat: 51003-M08H) at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (isoform Beta1) (Cat: 11609-H01H2) with a linear range of 0.018-0.125 μg/ml.2. Immobilized mouse ErbB3-His (Cat: 51003-M08H) at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 (aa 2-246)-Fc (Cat: 11609-H04H) with a linear range of 6.25-100 ng/ml.
HER3/ERBB3 Protein, Mouse, Recombinant (His Tag): Synonyms
C76256 Protein, Mouse; Erbb-3 Protein, Mouse; Erbb-3r Protein, Mouse; Erbb3 Protein, Mouse; Erbb3r Protein, Mouse; Her3 Protein, Mouse
HER3/ERBB3 Background Information
ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
erb-b2 receptor tyrosine kinase 3
Research Areas
- Cancer Drug Targets
- Receptor Tyrosine Kinases (RTKs)
References
- Kraus M.H., et al.,(1989), Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197.
- Plowman G.D., et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909.
- Katoh M., et al.,(1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.
Human HER3/ERBB3 Proteins
- HER3/ERBB3 Protein, Human, Recombinant (ECD, His Tag)10201-H08H
- HER3/ERBB3 Protein, Human, Recombinant10201-HCCH
- HER3/ERBB3 Protein, Human, Recombinant (His Tag), Biotinylated10201-H08H-B
- HER3/ERBB3 Protein, Human, Recombinant (hFc Tag), Biotinylated10201-H02H-B
- HER3/ERBB3 Protein, Human, Recombinant (ECD, hFc Tag)10201-H02H
- HER3/ERBB3 Protein, Human, Recombinant (mFc Tag)10201-H05H
- HER3/ERBB3 Protein, Human, Recombinant (aa 730-1065, His & GST Tag)10201-H20B1
Rat HER3/ERBB3 Proteins
- HER3/ERBB3 Protein, Rat, Recombinant (His Tag)80111-R08H
- HER3/ERBB3 Protein, Rat, Recombinant (hFc Tag)80111-R02H
Rhesus HER3/ERBB3 Proteins
- HER3/ERBB3 Protein, Rhesus, Recombinant (His Tag)90043-K08H
- HER3/ERBB3 Protein, Rhesus, Recombinant (hFc Tag)90043-K02H
- HER3/ERBB3 Protein, Rhesus, Recombinant90043-KCCH
- Development and characterization of small bispecific albumin-binding domains with high affinity for ErbB3AuthorNilvebrant, J;Astrand, M;Löfblom, J;Hober, S;Year2013JournalCell. Mol. Life Sci.PubMed ID: 23728098FoldedExpand
- Evaluating the Therapeutic Efficacy of Mono- and Bivalent Affibody-Based Fusion Proteins Targeting HER3 in a Pancreatic Cancer Xenograft ModelAuthorLeitao, CD;Rinne, SS;Altai, M;Vorontsova, O;Dunås, F;Jonasson, P;Tolmachev, V;Löfblom, J;Ståhl, S;Orlova, A;Year2020JournalPharmaceuticsPubMed ID: 32545760FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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