Sino Biological/FGFR1 Protein, Human, Recombinant (His & hFc Tag)/1/10616-H03H
商品编号：
10616-H03H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
FGFR1 Protein, Human, Recombinant (His & hFc Tag): Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 4-20 ng/mL
Protein Construction
A DNA sequence encoding the human FGFR1 (NP_075594.1) extracellular domain (Met 1-Glu 285) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Accession#
NP_075594.1
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Arg 22
Molecule Mass
The recombinant human FGFR1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 512 amino acids and has a predicted molecular mass of 57.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhFGFR1/Fc monomer is approximately 100-110 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
FGFR1 Protein, Human, Recombinant (His & hFc Tag): Images
Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 4-20 ng/mL
FGFR1 Protein, Human, Recombinant (His & hFc Tag): Synonyms
bFGF-R-1 Protein, Human; BFGFR Protein, Human; CD331 Protein, Human; CEK Protein, Human; FGFBR Protein, Human; FGFR-1 Protein, Human; FLG Protein, Human; FLT-2 Protein, Human; FLT2 Protein, Human; HBGFR Protein, Human; HH2 Protein, Human; HRTFDS Protein, Human; KAL2 Protein, Human; N-SAM Protein, Human; OGD Protein, Human
FGFR1 Background Information
FGFR1, also known as CD331, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. Fibroblast growth factors (FGFs) (FGF1 - 10 and 16 - 23) are mitogenic signaling molecules that have roles in angiogenesis, wound healing, cell migration, neural outgrowth and embryonic development. FGFs bind heparan sulfate glycosaminoglycans, which facilitates dimerization (activation) of FGF receptors. FGFR1 is a full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR1 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. CD331 can be detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. Defects in FGFR1 are a cause of Pfeiffer syndrome ，idiopathic hypogonadotropic hypogonadism， Kallmann syndrome type 2, osteoglophonic dysplasia and trigonocephaly non-syndromic.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
fibroblast growth factor receptor 1
Research Areas
- Cancer Drug Targets
- Receptor Tyrosine Kinases (RTKs)
References
- Schlessinger J, et al. (2000) Crystal structure of a ternary FGF-FGFR-heparin complex reveals a dual role for heparin in FGFR binding and dimerization. Mol Cell. 6(3):743-50.
- Dodé C, et al. (2007) Novel FGFR1 sequence variants in Kallmann syndrome, and genetic evidence that the FGFR1c isoform is required in olfactory bulb and palate morphogenesis. Hum Mutat. 28(1): 97-8.
- Kim HG, et al. (2005) Hypogonadotropic hypogonadism and cleft lip and palate caused by a balanced translocation producing haploinsufficiency for FGFR1. J Med Genet. 42(8):666-72.
Human FGFR1 Proteins
- FGFR1 Protein, Human, Recombinant (beta (IIIb), His Tag)16480-H08H
- FGFR1 Protein, Human, Recombinant (alpha (IIIb), His Tag)16482-H08H
- FGFR1 Protein, Human, Recombinant (His Tag), Biotinylated10616-H08H-B
- FGFR1 Protein, Human, Recombinant (ECD, hFc Tag)10616-H02H
- FGFR1 Protein, Human, Recombinant (His Tag)10616-H08H
- FGFR1 Protein, Human, Recombinant (alpha (IIIb), hFc Tag)16482-H02H
- FGFR1 Protein, Human, Recombinant (beta (IIIb), hFc Tag)16480-H02H
- FGFR1 Protein, Human, Recombinant (His & GST Tag)10616-H20B1
Mouse FGFR1 Proteins
- FGFR1 Protein, Mouse, Recombinant (His Tag)50186-M08H
- FGFR1 Protein, Mouse, Recombinant (hFc Tag)50186-M02H
Rhesus FGFR1 Proteins
- FGFR1 Protein, Rhesus, Recombinant (hFc Tag)90075-C02H
- FGFR1 Protein, Rhesus, Recombinant (His Tag)90075-C08H
- OM-RCA-01, a novel humanized monoclonal antibody targeting fibroblast growth factor receptor 1, in renal cell carcinoma modelAuthorI Tsimafeyeu, E Zaveleva, E Stepanova…Year2013JournalInvestigational new drugsApplicationELISAPubMed ID: 24026432FoldedExpand
If you couldn"t find the specific citation, you can:
- Find All Citations
- Submit a CitationGet a Publication Reward
- Leave Us a Message
E.coli protein expression service
Mammalian transient protein expression service
Baculovirus-insect expression service
Stable cell line development service (CHO / HEK293)
品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
联络我们
