Sino Biological/PCSK1 Protein, Human, Recombinant (His Tag)/1/10129-H08H
商品编号：
10129-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PCSK1 Protein, Human, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human PCSK1 (NP_000430.3) (Met 1-Arg 617) was fused with a polyhistidine tag at the C-terminus.
Accession#
NP_000430.3
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ser 111
Molecule Mass
The purified recombinant human PCSK1 consists of 518 amino acids after removal of the pro peptide due to autocatalytic processing, corresponding to the active form (aa 111-617), and has a predicted molecular mass of 57.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the active rh PCSK1 is approximately 66 kDa due to glycosylation.
Formulation
Lyophilized from sterile 25mM Tris, 150mM NaCl, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
PCSK1 Protein, Human, Recombinant (His Tag): Images
PCSK1 Protein, Human, Recombinant (His Tag): Synonyms
BMIQ12 Protein, Human; NEC1 Protein, Human; PC1 Protein, Human; PC3 Protein, Human; SPC3 Protein, Human
PCSK1 Background Information
Neuroendocrine convertase 1, also known as Prohormone convertase 1, Proprotein convertase 1, PCSK1 and NEC1, is an enzyme which belongs to the peptidase S8 family and Furin subfamily. PCSK1 is an enzyme that performs the proteolytic cleavage of prohormones to their intermediate (or sometimes completely cleaved) forms. It is present only in neuroendocrine cells such as brain, pituitary and adrenal, and most often cleaves after a pair of basic residues within prohormones but can occasionally cleave after a single arginine. It binds to a protein known as proSAAS, which also represents its endogenous inhibitor. PCSK1 is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK1 substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency). PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction. It is due to impaired processing of prohormones.
Full Name
proprotein convertase subtilisin/kexin type 1
Research Areas
- Serine Proteases and Regulators
References
- JacksonRS. et al., 2003, J. Clin. Invest. 112:1550-60.
- FarooqiI.S. et al., 2007, J. Clin. Endocrinol. Metab. 92: 3369-73.
- Benzinou,M.et al., 2008,Nat Genet. 40(8):943-5.
- BenzinouM. et al., 2008, Nat. Genet. 40:943-5.
- RenströmF, et al., 2009, Hum. Mol. Genet. 18 (8): 1489-96.
- Effects of rs6234/rs6235 and rs6232/rs6234/rs6235 PCSK1 single-nucleotide polymorphism clusters on proprotein convertase 1/3 biosynthesis and activityAuthorMbikay, M;Sirois, F;Nkongolo, KK;Basak, A;Chrétien, M;Year2011JournalMol. Genet. Metab.PubMed ID: 22000902FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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