Anti-His tag Antibody, Mouse Monoclonal General Information
Anti-His tag Antibody, Mouse Monoclonal Validated Applications
| Application | Dilution |
|---|---|
| WB | 1/1000-1/10000 |
Anti-His tag Antibody, Mouse Monoclonal Images
Detection Limit
Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.
Sample:Recombiant Protein
Lane A: His-Human PA2G4(10ng)
Lane B: His-Human PA2G4(5ng)
Lane C: His-Human PA2G4(2ng)
Lane D: His-Human PA2G4(1ng)
Lane E: His-Human PA2G4(0.5ng)
Lane F: His-Human PA2G4(0.2ng)
Lane G: Human B7-2-His(10ng)
Lane H: Human B7-2-His(5ng)
Lane I: Human B7-2-His(2ng)
Lane J: Human B7-2-His(1ng)
Lane K: Human B7-2-His(0.5ng)
Lane L: Human B7-2-His(0.2ng)
Secondary
Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Recognize the protein with 10×His tag.
Anti-10×His Tag mouse monoclonal antibody at 1:1000 dilution.
Sample:Recombiant Protein
Lane A: Human IL2Ra-His(50ng)
Lane B: Human IL6R-His(50ng)
Lane C: Human AZGP1-His(100ng)
Lane D: Human ART3-His(20ng)
Lane E: Human LILRA3-His(20ng)
Secondary
Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Recognize the protein with 6×His tag.
Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.
Sample:Recombiant Protein
Lane A: His-Mouse TNFSF9(50ng)
Lane B: His-Human AKR1C2(200ng)
Lane C: His-Human LYPLA2(50ng)
Lane D: Human MOG-His(100ng)
Secondary
Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.
Sample:Recombiant Protein
Lane A: Human IL2Ra-His(50ng)
Lane B: Human IL6R-His(50ng)
Lane C: Human AZGP1-His(100ng)
Lane D: Human ART3-His(20ng)
Lane E: Human LILRA3-His(20ng)
Secondary
Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Negative binding protein without His tag.
Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.
Sample:Recombiant Protein
Lane A: Human C100RF54-Fc(100ng)
Lane B: Rat B7H5-Fc(100ng)
Lane C: Rhesus C9H10orf54-Fc(100ng)
Lane D: S3-Mouse ALK6-Fc(100ng)
Lane E: Human LAG3-Fc(100ng)
Lane F: GST-Human MKI67(100ng)
Lane G: GST-Human CD247(100ng)
Lane H: MYC-GST(100ng)
Secondary
Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Recognize protein with His tag (internal).
Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.
Sample:Recombiant Protein
Lane A: TrxA-His-EK-Human APOE(100ng)
Lane B: TrXA-His-EK-Human BCL6(100ng)
Lane C: Trx-His-EK-Human SOCS3(100ng)
Secondary
Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Recognize His tag at C-terminal of the protein.
Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.
Sample:Recombiant Protein
Lane A: Human LRG1-His(50ng)
Lane B: Human 4-1BB-His(50ng)
Lane C: Human SLAMF1-His(50ng)
Lane D: Human ALPL-His(100ng)
Lane E: Human IL2Ra-His(50ng)
Lane F: Human IL6R-His(50ng)
Lane G: Human AZGP1-His(100ng)
Secondary
Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Recognize His tag at N-terminal of the protein.
Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.
Sample:Recombiant Protein
Lane A: His-Human TPO(100ng)
Lane B: His-Mouse CLEC7A(200ng)
Lane C: His-Human LYPLA2(50ng)
Lane D: His-Human COCH(200ng)
Lane E: His-Mouse TFRC(100ng)
Lane F: His-Human HSPA1A(100ng)
Lane G: His-Human PA2G4(50ng)
Secondary
Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Recognize His tag fusion proteins which expressed by Baculovirus.
Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.
Lane A: Negative control
Lane B: His-Human LYPLA2 transfected Baculovirus Lysate(2ug)
Lane C: Human AZGP1-His transfected Baculovirus Lysate(2ug)
Lane D: Human IL2Ra-His transfected Baculovirus Lysate(2ug)
Secondary
Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Recognize His tag fusion proteins which expressed by E. coli.
Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.
Lane A: Negative control
Lane B: Human M2E1-His transfected E.coli Lysate(0.5ug)
Lane C: His-sSPA transfected E.coli Lysate(0.5ug)
Lane D: His-Human GH1 transfected E.coli Lysate(0.5ug)
Secondary
Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Recognize His tag fusion proteins which expressed by 293 cell.
Anti-6×His Tag mouse monoclonal antibody at 1:1000 dilution.
Lane A: Negative control
Lane B: His-Human LYPLA2 transfected 293 Cell Lysate(2ug)
Lane C: His-Human AKR1C2 transfected 293 Cell Lysate(2ug)
Lane D: Human LILRA3-His transfected 293 Cell Lysate(2ug)
Secondary
Rabbit Anti-Mouse IgGF(ab)2/HRP at 1:10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
His tag Background Information
- Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
- Lin Z, et al. (2015) Aggregating tags for column-free protein purification. Biotechnol J 10 (12): 1877-1886.
- Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.
- Recombinant baculovirus BacCarassius-D4ORFs has potential as a live vector vaccine against CyHV-2AuthorLi, K;Yuan, R;Zhang, M;Zhang, T;Gu, Y;Zhou, Y;Dai, Y;Fang, P;Feng, Y;Hu, X;Cao, G;Xue, R;Chen, H;Gong, C;Year2019JournalFish & Shellfish ImmunologyApplicationWBPubMed ID: 31163296FoldedExpand
- 微小隐孢子虫 Ⅰ 型跨膜蛋白 Cgd7_4560 的亚细胞定位及其对 HCT-8 细胞的黏附特性Author邵清延, ;王科杰, ;王东强, ;张天宇, ;焦新, ;Year2019Journal吉林农业大学ApplicationWBPubMed ID: 31214999FoldedExpand
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