Anti-GAPDH Antibody, Rabbit Polyclonal General Information
Anti-GAPDH Antibody, Rabbit Polyclonal Validated Applications
| Application | Dilution |
|---|---|
| WB | 1:500-1:2000 |
| ELISA | 1:5000-1:10000 |
| IP | 0.5-2 μL/mg of lysate |
Anti-GAPDH Antibody, Rabbit Polyclonal Images
-HD09MY2917-IP.png)
GAPDH was immunoprecipitated using:
Lane A:0.5 mg HepG2 Whole Cell Lysate
Lane B:0.5 mg Jurkat Whole Cell Lysate
Lane C:0.5 mg Hela Whole Cell Lysate
4 µL anti-GAPDH rabbit polyclonal antibody and 15 μl of50 %Protein G agarose.
Primary antibody:
Anti-GAPDH rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
-HD09MY2917-WB.png)
Anti-GAPDH rabbit polyclonal antibody at 1:500 dilution
Lane A: HepG2 whole cell lysate
Lane B: Jurkat whole cell lysate
Lane C: Hela whole cell lysate
Lane D: A431 whole cell lysate
Lysates/proteins at 30 μg per lane.
Secondary
Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:36 kDa
Observed band size:36 kDa
(We are unsure as to the identity of these extra bands.)
Anti-GAPDH Antibody, Rabbit Polyclonal: Synonyms
GAPDH Background Information
- Adaptor Proteins
- Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
- Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
- Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
- Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
- Uric acid upregulates the adiponectin‑adiponectin receptor 1 pathway in renal proximal tubule epithelial cellsAuthorYang, Q;Fu, C;Xiao, J;Ye, Z;Year2017JournalMolecular Medicine ReportsApplicationWBPubMed ID: 29359786FoldedExpand
- Serum anti‑GAPDH autoantibody levels reflect the severity of cervical lesions: A potential serum biomarker for cervical cancer screeningAuthorXu, M;Kim, H;Kim, S;Ju, W;Kim, Y;Chang, K;Kim, H;Year2019JournalOncol LettApplicationWBPubMed ID: 31289495FoldedExpand
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