Anti-GST tag Antibody, Mouse Monoclonal General Information
Anti-GST tag Antibody, Mouse Monoclonal Validated Applications
| Application | Dilution |
|---|---|
| WB | 1/1000-1/10000 |
| IP | 1-4 μl/mg of lysate |
Anti-GST tag Antibody, Mouse Monoclonal Images
Anti-GST Tag was immunoprecipitated using:
Lane A: GST-ARG1-Flag transfected 293 cell lysate (0.5mg)
Lane B: Flag-ARG1-GST transfected 293 cell lysate (0.5mg)
2 μg anti-HA Tag mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.
Primary antibody:
Anti-GST Tag rabbit monoclonal antibody, at 1:100 dilution
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Recognize GST tag fusion protein human ERK2.
Anti-GST Tag mouse monoclonal antibody at 1:4000
Lane A: GST-ERK2 (Recombinant protein, Cat#10030-H09B) (100ng)
Lane B: GST-ERK2 (Recombinant protein, Cat#10030-H09B) (50ng)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Recognize GST tag fusion protein human USP7.
Anti-GST Tag mouse monoclonal antibody at 1:4000
Lane A: GST-USP7 (Recombinant protein, Cat#11681-H20B) (100ng)
Lane B: GST-USP7 (Recombinant protein, Cat#11681-H20B) (50ng)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Recognize GST tag fusion proteins which expressed by 293 cell.
Anti-GST Tag mouse monoclonal antibody at 1:1250 or 1:2500
Lane A: GST-FABP4-flag transfected 293 Cell Lysate (30ug)
Lane B: flag-FABP4-GST transfected 293 Cell Lysate (30ug)
Lane C: flag-AGR1-GST transfected 293 Cell Lysate (30ug)
Lane D: Negative control
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Recognize GST tag fusion proteins which expressed by E. coli.
Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250
Lane A: GST-mBID transfected E.coli cell lysate (0.5ug)
Lane B: GST-mBID transfected E.coli cell lysate (0.2ug)
Lane C: GST-mBID transfected E.coli cell lysate (0.1ug)
Lane D: GST-mBID transfected E.coli cell lysate (0.05ug)
Lane E: GST-mBID transfected E.coli cell lysate (0.02ug)
Lane F: GST-YWHAB transfected E.coli cell lysate (0.5ug)
Lane G: GST-YWHAB transfected E.coli cell lysate (0.2ug)
Lane H: GST-YWHAB transfected E.coli cell lysate (0.1ug)
Lane I: GST-YWHAB transfected E.coli cell lysate (0.05ug)
Lane J: GST-YWHAB transfected E.coli cell lysate (0.02ug)
Lane K: Empty vecter (0.5ug)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:1000
Lane A: GST-mBID transfected E.coli cell lysate (0.1ug)
Lane B: GST-YWHAB transfected E.coli cell lysate (0.1ug)
Lane C: Empty vecter (0.1ug)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:25000
Lane A: GST-mBID transfected E.coli cell lysate (0.2ug)
Lane B: GST-YWHAB transfected E.coli cell lysate (0.2ug)
Lane C: Empty vecter (0.2ug)
Secondary
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
GST tag Background Information
- Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
- Monti M, et al. (2007) Functional proteomics: Protein-protein interactions in vivo. Ital J Biochem 56 (4): 310-314.
- Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.
- 微小隐孢子虫 Ⅰ 型跨膜蛋白 Cgd7_4560 的亚细胞定位及其对 HCT-8 细胞的黏附特性Author邵清延, ;王科杰, ;王东强, ;张天宇, ;焦新, ;Year2019Journal吉林农业大学PubMed ID: 31214999FoldedExpand
- Neddylation-mediated Nedd4-2 activation regulates ubiquitination modification of renal NBCe1AuthorTu, J;Zhang, B;Fang, G;Chang, W;Zhao, Y;Year2020JournalExp. Cell Res.PubMed ID: 32173470FoldedExpand
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