Sino Biological/Human CD28 Antibody/1/11524-H001
商品编号：
11524-H001
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Human CD28 Antibody General Information
Product name
Human CD28 Antibody
Validated applications
FCM,Func/A
Species reactivity
Reacts with: Human
Specificity
Human CD28
Source
Human IgG2 Clone #H001
Formulation
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Conjugate
Unconjugated
Endotoxin
< 0.5="">
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Human CD28 Antibody Validated Applications
| Application | Dilution |
|---|---|
| FCM | 0.5-2 μg/Test |
Notes
| Agonist Activity (Func/A)： | Agonist Activity – Measured by its ability to induce proliferation of human T cells. The ED50 for this effect is typically 0.2-1 ug/mL. |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Human CD28 Antibody Images
The staining effect of Human CD28 antibody to CD28 of Human Peripheral Blood Lymphocytes by Flow cytometric analysis. A: Lymphocytes were stained with PE-conjugated humanized CD28 antibody. B: The binding effect of PE-conjugated human CD28 antibody(Red histograms) in the lymphocytes can be blocked by pre-incubation of the CD28 antibody (BD, 555725) for 20min (blue histograms).
CD28+ T cell staining of lymphocytes using human CD28 antibody by Flow cytometric analysis. Lymphocytes were stained with PE-conjugated humanized CD28 antibody and CD19-APC (BD, 555415).
Effect of human CD28 antibody on stimulating IL-2 secretion of CD4 T cells co-activated with CD3 antibody. The isolated CD4+ T cells (1e5/well) were cultured in the 96-well cell plates pre-coated with 2 μg/mL human CD3 (humanized OKT3) antibody (GMP-10977-H001) (100 μL/well) in the culture medium containing serial dilutions of CD28 antibody. 48 hours later, IL-2 secretion in the medium was measured by IL-2 Quantitative ELISA Kit.
Human CD28 Antibody: Synonyms
Anti-Tp44 Antibody
CD28 Background Information
CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
- Cancer Immunotherapy
- Co-stimulatory Immune Checkpoint Targets
- Immune Checkpoint
- Immune Checkpoint Detection: Antibodies
- Immune Checkpoint Detection: ELISA Antibodies
- Immune Checkpoint Detection: FCM Antibodies
- Immune Checkpoint Detection: IHC Antibodies
- Immune Checkpoint Detection: WB Antibodies
- Immune Checkpoint Targets
- Immunotherapy
- Targeted Therapy
Full Name
CD28 molecule
Research Areas
- ITIM/ITAM Immunoreceptors and Related Molecules
- Cancer Drug Targets
Related Pathways
T Cell Receptor Signaling Pathway
References
- Keir ME, et al. (2005) The B7/CD28 costimulatory family in autoimmunity. Immunol Rev. 204: 128-43.
- Sansom DM, et al. (2006) The role of CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4) in regulatory T-cell biology. Immunol Rev. 212: 131-48.
- Bjrgo E, et al. (2010) Novel mechanism of signaling by CD28. Immunol Lett. 129(1): 1-6.
- The role of Th1 / Th2 Cytokines played in Regulation of Specific CD4 + Th1 Cells Conversion and Activation during Inflammatory Reaction of Chronic Obstructive Pulmonary DiseaseAuthorSun, J;Liu, T;Yan, Y;Huo, K;Zhang, W;Liu, H;Shi, Z;Year2018JournalScand. J. Immunol.PubMed ID: 29752829FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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