Anti-TNF-alpha Antibody, Rabbit Polyclonal General Information
Anti-TNF-alpha Antibody, Rabbit Polyclonal Validated Applications
| Application | Dilution |
|---|---|
| WB | 1:500-1:2000 |
| ELISA | 1:5000-1:10000 |
| IP | 1-4 μL/mg of lysate |
Anti-TNF-alpha Antibody, Rabbit Polyclonal Images
-HB06OC1912-IP.png)
TNFa was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
Lane B:0.5 mg HepG2 Whole Cell Lysate
Lane C:0.5 mg SW480 Whole Cell Lysate
2 µL anti-TNFa rabbit polyclonal antibody and 15 μl of50 %Protein G agarose.
Primary antibody:
Anti-TNFa rabbit polyclonal antibody,at 1:200 dilution
Secondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 33 kDa
Anti-TNF-alpha rabbit polyclonal antibody at 1:500 dilution
Lane A: HepG2 Whole Cell Lysate
Lysates/proteins at 30 μg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800)at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:25 kDa
Observed band size:27 kDa
Anti-TNF-alpha Antibody, Rabbit Polyclonal: Synonyms
TNF-alpha Background Information
TNF-alpha Protein & Antibody- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
- Cancer Drug Targets
- Neuroinflammation
Death Receptor Signaling
NF-kB (NFkB) Pathway
IL17 signaling pathway
- Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
- Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
- ADAM-17 is a poor prognostic indicator for patients with hilar cholangiocarcinoma and is regulated by FoxM1AuthorJiao, X;Yu, W;Qian, J;Chen, Y;Wei, P;Fang, W;Yu, G;Year2018JournalBMC CancerApplicationWBPubMed ID: 29776401FoldedExpand
If you couldn"t find the specific citation, you can:
- Find All Citations
- Submit a CitationGet a Publication Reward
- Leave Us a Message
Recombinant Antibody Production Service
High-throughput Recombinant Antibody Production
Large-scale Antibody Production
Antibody Development Service
Rabbit MAb Production Service
Mouse MAb Production Service
Rabbit PAb Production Service
Phospho-Specific Antibody
Anti-Idiotype Antibody
Fast Antibody Development Service
Mouse MAb
Rabbit PAb
More Antibody Production Service
Hybridoma Antibody Production
Chimeric Antibody
Antibody Fragment
Bispecific Antibody
We also have Immunoassay Service. Click to see more.
