Sino Biological/Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal/1/10140-R014
商品编号：
10140-R014
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal General Information
Product name
Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal
Validated applications
ELISA(Det),IHC-P,FCM,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human L1CAM
Immunogen
Recombinant Human CD171 / N-CAML1 / L1CAM protein (Catalog#10140-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD171 / N-CAML1 / L1CAM (rh CD171 / N-CAML1 / L1CAM; Catalog#10140-H08H; NP_000416.1; Met1-Glu1120).
Source
Monoclonal Rabbit IgG Clone #014
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal Validated Applications
| Application | Dilution |
|---|---|
| IHC-P | 1:100-1:500 |
| ICC-IF | 1:20-1:100 |
| FCM | 1:25-1:100 |
| ELISA(Det) | 1:1000-1:10000 |
Notes
| ELISA(Det)： | This antibody will detect Human CD171 / L1CAM in ELISA pair set (Catalog: # SEK10140). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10140-R001). |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal Images
Immunochemical staining of human CD171 in human appendix with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to myenteric nerve plexus.
Flow cytometric analysis of human CD171 expression on HeLa cells. Cells were stained with purified anti-Human CD171, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescence staining of Human CD171 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD171 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.
Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal: Synonyms
Anti-CAML1 Antibody; Anti-CD171 Antibody; Anti-HSAS Antibody; Anti-HSAS1 Antibody; Anti-MASA Antibody; Anti-MIC5 Antibody; Anti-N-CAM-L1 Antibody; Anti-N-CAML1 Antibody; Anti-NCAM-L1 Antibody; Anti-S10 Antibody; Anti-SPG1 Antibody
L1CAM Background Information
L1 cell adhesion molecule (L1CAM), also designated as CD171, is a cell adhesion receptor of the immunoglobulin superfamily, known for its roles in nerve cell function. While originally believed to be present only in brain cells, in recent years L1-CAM has been detected in other tissues, and a variety of cancer cells, including some common types of human cancer. L1CAM interacts with a variety of ligands including axonin-1, CD9, neurocan, and integrins, and it has been revealed that the RGD motif in the sixth Ig domain of L1CAM is a binding site for integrins, thus important for nuclear signaling. Disruption of L1CAM function causes three X-linked neurological syndromes, i.e. hydrocephalus, MASA syndrome (mental retardation, aphasia, shuffling gait, and adducted thumbs), and spastic paraplegia syndrome. Overexpression of L1CAM in normal and cancer cells increased motility, enhanced growth rate, and promoted cell transformation and tumorigenicity. Recent work has identified L1CAM (CD171) as a novel marker for human carcinoma progression, and a candidate for anti-cancer therapy.
Full Name
L1 cell adhesion molecule
References
- Meier F, et al. (2006) The adhesion molecule L1 (CD171) promotes melanoma progression. Int J Cancer. 119(3): 549-55.
- Gavert N, et al. (2008) L1-CAM in cancerous tissues. Expert Opin Biol Ther. 8(11): 1749-57.
- Issa Y, et al. (2009) Enhanced L1CAM expression on pancreatic tumor endothelium mediates selective tumor cell transmigration. J Mol Med. 87(1): 99-112.
- Weidle UH, et al. (2009) L1-CAM as a target for treatment of cancer with monoclonal antibodies. Anticancer Res. 29(12): 4919-31.
- Raveh S, et al. (2009) L1 cell adhesion molecule (L1CAM) in invasive tumors. Cancer Lett. 282(2): 137-45.
- Wolterink S, et al. (2010) Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer Res. 70(6): 2504-15.
- Manufacture of CAR-T Cells from Mobilized Cyropreserved Peripheral Blood Stem Cell Units Depends on Monocyte DepletionAuthorKünkele, A;Brown, C;Beebe, A;Mgebroff, S;Johnson, AJ;Taraseviciute, A;Rolczynski, LS;Chang, CA;Finney, OC;Park, JR;Jensen, MC;Year2018JournalBiol. Blood Marrow Transplant.ApplicationFCMPubMed ID: 30315942FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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