Sino Biological/Anti-PARP Antibody, Rabbit Polyclonal/1/100984-T46
商品编号：
100984-T46
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Anti-PARP Antibody, Rabbit Polyclonal General Information
Product name
Anti-PARP Antibody, Rabbit Polyclonal
Validated applications
WB,IHC-P,ICC/IF,IP
Species reactivity
Reacts with: Human
Specificity
Human PARP
Immunogen
A synthetic peptide corresponding to the center region of the Human PARP-1 / PARP
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PARP-1 / PARP, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-PARP Antibody, Rabbit Polyclonal Validated Applications
| Application | Dilution |
|---|---|
| WB | 1:500-1:2000 |
| IHC-P | 1:1000-1:4000 |
| ICC-IF | 1:200-1:1000 |
| IP | 1-4 μL/mg of lysate |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Anti-PARP Antibody, Rabbit Polyclonal Images
-HD09FE2722-1ug-HeLa-IF.png)
Immunofluorescence staining of PARP1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human PARP1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to nucleus.
-HD09FE2722-0.5ug-placenta-IHC-P.png)
Immunochemical staining of human PARP1 in human placenta with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
-HD09FE2722-0.5ug-brain-IHC-P.png)
Immunochemical staining of human PARP1 in human brain with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
-HD09FE2722-IP.png)
PARP1 was immunoprecipitated using:
Lane A:0.5 mg Raji Whole Cell Lysate
4 µL anti-PARP1 rabbit polyclonal antibody and 15 μl of50 %Protein G agarose.
Primary antibody:
Anti-PARP1 rabbit polyclonal antibody,at 1:100 dilution
Secondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.
Performed under reducing conditions.
Predicted band size: 113 kDa
Observed band size: 113 kDa
-HD09FE2722-WB.png)
Anti-PARP1 rabbit polyclonal antibody at 1:500 dilution
Lane A: JurkatWhole Cell Lysate
Lane B: RajiWhole Cell Lysate
Lysates/proteins at 30 μg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800)at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:113 kDa
Observed band size:120 kDa
(We are unsure as to the identity of these extra bands.)
Anti-PARP Antibody, Rabbit Polyclonal: Synonyms
Anti-ADPRT Antibody; Anti-ADPRT1 Antibody; Anti-ARTD1 Antibody; Anti-pADPRT-1 Antibody; Anti-PARP Antibody; Anti-PARP-1 Antibody; Anti-PPOL Antibody
PARP Background Information
Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2"-position of the terminal adenosine moiety, building up a polymer with an average chain length of 2-3 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrated to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors are thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
poly (ADP-ribose) polymerase 1
Research Areas
- Cancer Drug Targets
References
- Malanga M. et al., 1998, J Biol Chem. 273: 11839-11843.
- Ariumi Y. et al., 1999, Oncogene. 18: 4616-4625.
- Helleday T. et al., 2005, Cell Cycle. 4: 1176-1178.
- Ahell I. et al., 2008, Nature. 451: 81-85.
- Synergistic suppression effect on tumor growth of acute myeloid leukemia by combining cytarabine with an engineered oncolytic vaccinia virusAuthorPeng, J;Wang, S;Fan, W;Li, S;Wu, Y;Mou, X;Wang, J;Tong, X;Year2018JournalOnco Targets TherApplicationWBPubMed ID: 30410347FoldedExpand
- Enhancing the antitumor activity of an engineered TRAIL-coated oncolytic adenovirus for treating acute myeloid leukemiaAuthorWang, Z;Liu, W;Wang, L;Gao, P;Li, Z;Wu, J;Zhang, H;Wu, H;Kong, W;Yu, B;Yu, X;Year2020JournalSig Transduct Target TherApplicationWBFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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