Sino Biological/Anti-H7N9 Hemagglutinin / HA Antibody, Hemagglutination Inhibition Activity, Rabbit MAb/1/11082-R002
商品编号：
11082-R002
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Anti-H7N9 Hemagglutinin / HA Antibody, Hemagglutination Inhibition Activity, Rabbit MAb General Information
Product name
Anti-H7N9 Hemagglutinin / HA Antibody, Hemagglutination Inhibition Activity, Rabbit MAb
Validated applications
ELISA,ELISA(Cap),FCM,HemagglutininInhibition(HI)
Application notes
(Antibody"s applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
H7N9 Hemagglutinin/HAHas cross-reactivity in ELISA withH7N9 (A/Hangzhou/1/2013) HAH7N9 (A/Shanghai/2/2013) HAH7N9 (A/Hangzhou/3/2013) HAH7N9 (A/Shanghai/1/2013) HAH7N9 (A/Anhui/1/2013) HAH7N9 (A/Pigeon/Shanghai/S1069/2013) HAH7N9 (A/Shanghai/4664T/2013) HAH7N9 (A/Zhejiang/DTID-ZJU10/2013) HAH7N9 (A/Zhejiang/1/2013) HAH7N3 (A/turkey/Italy/214845/2002) HAH7N7 (A/Netherlands/219/03) HAH7N7 (A/chicken/Netherlands/1/03) HAH7N7 (A/equine/Kentucky/1a/1975) HAH7N8 (A/mallard/Netherlands/33/2006) HAH7N1 (A/turkey/Italy/4602/99) HANo cross-reactivity in ELISA withH1N1 (A/California/07/2009) HAH2N2 (A/Canada/720/2005) HAH3N2 (A/Brisbane/10/2007) HAH4N6 (A/mallard/Ohio/657/2002) HAH5N1 (A/Anhui/1/2005) HAH6N1 (A/northern shoveler/California/HKWF115/2007) HAH8N4 (A/pintail duck/Alberta/114/1979) HAH9N2 (A/Hong Kong/1073/99) HAH10N3 (A/duck/Hong Kong/786/1979) HAH11N2 (A/duck/Yangzhou/906/2002) HAH12N5 (A/green-winged teal/ALB/199/1991) HAH13N8 (A/black-headed gull/Netherlands/1/00) HAH15N8 (A/duck/AUS/341/1983) HAH16N3 (A/black-headed gull/Sweden/5/99) HA
Source
Monoclonal Rabbit IgG Clone #002
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Anti-H7N9 Hemagglutinin / HA Antibody, Hemagglutination Inhibition Activity, Rabbit MAb Validated Applications
| Application | Dilution |
|---|---|
| ELISA | 0.1-0.2 μg/mL |
| FCM | 0.5-2 μg/Test |
| ELISA(Cap) | 0.5-4 µg/ml |
Notes
| ELISA(Cap)： | This antibody will detect Influenza A H7N9 HA / Hemagglutinin in ELISA pair set (Catalog: # SEK11082). In a sandwich ELISA, it can be used as capture antibody. |
| Hemagglutinin Inhibition (HI)： | Serially diluted antibody was pre-incubated with 4 units of hemagglutination titer of HA proteins for 1 hr at room temperature, the concentraion of antibody to stop 1% Guinea pig red cells hemagglutination is measured. |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Anti-H7N9 Hemagglutinin / HA Antibody, Hemagglutination Inhibition Activity, Rabbit MAb Images
Flow cytometric analysis of Purified anti-H7N9-HA antibody on 293 transfected cells. 293 cells were transfected with plasmid DNA of H7N7 (A/Netherlands/219/2003) Hemagglutinin (Cat.No. VG11082-G-N), then the cells were collected at 72 hours posttransfection, and the cell surface expression of HA were measured by flowcytometry.The transfected cells stained with Purified Rabbit anti-H7N9-HA ( Bold line hisgram, 1 μg /test), To demonstrate specificity of staining, the binding of 11082-R002 was blocked by the preincubation of the purified antibody with molar excess of recombinant Influenza A H7N9 (A/Shanghai/1/2013) Hemagglutinin (Left panel, 5 μg,Cat. No. 40104-V08H),and Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin (Right panel, 5 μg, Cat. No. 40103-V08H) for 1 hour (Dashed line hisgram), then stained with a FITC-conjugated second step antibody, grey line histogram represented negative control.
Hemagglutinin/HA Background Information
The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells" sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
References
- White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
- Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
- Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
- Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018
- A novel eight amino acid insertion contributes to the hemagglutinin cleavability and the virulence of a highly pathogenic avian influenza A (H7N3) virus in miceAuthorSun, X;Belser, JA;Tumpey, TM;Year2016JournalVirologyApplicationWBPubMed ID: 26629952FoldedExpand
- Generation of Stable Influenza Virus Hemagglutinin through Structure-Guided RecombinationAuthorTsai, CH;Wei, SC;Jan, JT;Liao, LL;Chang, CJ;Chao, YC;Year2019JournalACS Synth BiolApplicationELISAPubMed ID: 31565926FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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