Sino Biological/Anti-Histone H3 Antibody, Mouse Monoclonal/1/100005-MM01-免疫在线蚂蚁淘旗下平台

当前位置：首页 > 产品中心 > juhewu > Sino Biological/Anti-Histone H3 Antibody, Mouse Monoclonal/1/100005-MM01

商品详细Sino Biological/Anti-Histone H3 Antibody, Mouse Monoclonal/1/100005-MM01

Sino Biological/Anti-Histone H3 Antibody, Mouse Monoclonal/1/100005-MM01

商品编号： 100005-MM01

品牌： sinobiological

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：聚合物

公司分类： juhewu

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Description
Related Products
Citations
Antibody Production Service

Anti-Histone H3 Antibody, Mouse Monoclonal General Information

Product name

Anti-Histone H3 Antibody, Mouse Monoclonal

Validated applications

WB,IHC-P

Species reactivity

Reacts with: Human

Specificity

Human Histone H3

Immunogen

A synthetic peptide corresponding to the C-terminus of the human Histone H3.

Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human Histone H3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Source

Monoclonal Mouse IgG2b Clone #1F6D7C4

Purification

Protein A

Formulation

0.2 μm filtered solution in PBS

Conjugate

Unconjugated

Form

Liquid

Shipping

This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Histone H3 Antibody, Mouse Monoclonal Validated Applications

Application	Dilution
WB	1/1000-1/100000
IHC-P	1/50-1/1000

Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Histone H3 Antibody, Mouse Monoclonal Images

Human Histone H3 Immunohistochemistry(IHC) 4056

Immunochemical staining of human histone H3 in human mammary gland with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus.

Human Histone H3 Immunohistochemistry(IHC) 4057

Immunochemical staining of human histone H3 in human colon carcinoma with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (polypeptide).

Human Histone H3 Western blot (WB) 13023

Anti-Histone H3 mouse monoclonal antibody at 1:2000 dilution

Lane A: Hela Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: 293 Whole Cell Lysate

Lysates/proteins at 30 μg per Lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/7500 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size: 18 kDa

Human Histone H3 Western blot (WB) 20381

Anti-Histone H3 mouse monoclonal antibody at 1:10000, 1:20000, 1:50000 dilution

Lysates/proteins at 30 μg per Lane.

Secondary

Rabbit Anti-Mouse IgG F(ab)2/HRPat 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size:18 kDa

Human Histone H3 Western blot (WB) 20382

Anti-Histone H3 mouse monoclonal antibody at 1:20000 dilution

Lysates/proteins at 30 μg per Lane.

Secondary

Rabbit Anti-Mouse IgG F(ab)2/HRPat 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size:18 kDa

Human Histone H3 Western blot (WB) 20383

Anti-Histone H3 mouse monoclonal antibody at 1:20000 dilution

Lysates/proteins at 30 μg per Lane.

Secondary

Rabbit Anti-Mouse IgG F(ab)2/HRPat 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size:18 kDa

Human Histone H3 Western blot (WB) 20384

Anti-Histone H3 mouse monoclonal antibody at 1:20000 dilution

Lysates/proteins at 30 μg per Lane.

Secondary

Rabbit Anti-Mouse IgG F(ab)2/HRPat 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size:18 kDa

Human Histone H3 Western blot (WB) 21458

Anti-Histone H3 mouse monoclonal antibody at 1:1000 dilution

Lane A: Histone H3 konckout Hela Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 10 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:18 kDa

Observed band size:18 kDa

Histone H3 Background Information

Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the "beads on a string" structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes.

References

Bhasin M, et al., 2006, 13(1): 102-12.
Lachner M, et al., 2001, 410(6824): 116-20.
Rosenfeld JA, et al., 2009, BMC Genomics. 10: 143.

Anti-Histone H3 Antibody
【Mouse】【Monoclonal】【ICC/IF】
100002-MM02

Triazole-dithiocarbamate based selective lysine specific demethylase 1 (LSD1) inactivators inhibit gastric cancer cell growth, invasion, and migration
Author
Zheng, YC;Duan, YC;Ma, JL;Xu, RM;Zi, X;Lv, WL;Wang, MM;Ye, XW;Zhu, S;Mobley, D;Zhu, YY;Wang, JW;Li, JF;Wang, ZR;Zhao, W;Liu, HM;
Year
2013
Journal
J. Med. Chem.
PubMed ID: 24131029FoldedExpand
Design, Synthesis, and Structure-Activity Relationship of Novel LSD1 Inhibitors Based on Pyrimidine-Thiourea Hybrids As Potent, Orally Active Antitumor Agents
Author
Ma, LY;Zheng, YC;Wang, SQ;Wang, B;Wang, ZR;Pang, LP;Zhang, M;Wang, JW;Ding, L;Li, J;Wang, C;Hu, B;Liu, Y;Zhang, XD;Wang, JJ;Wang, ZJ;Zhao, W;Liu, HM;
Year
2015
Journal
J. Med. Chem.
Application
WB
PubMed ID: 25610955FoldedExpand
Point mutation impairs centromeric CENH3 loading and induces haploid plants
Author
Karimi-Ashtiyani, R;Ishii, T;Niessen, M;Stein, N;Heckmann, S;Gurushidze, M;Banaei-Moghaddam, AM;Fuchs, J;Schubert, V;Koch, K;Weiss, O;Demidov, D;Schmidt, K;Kumlehn, J;Houben, A;
Year
2015
Journal
Proc. Natl. Acad. Sci. U.S.A.
Application
WB
PubMed ID: 26294252FoldedExpand
The Tet1 and histone methylation expression pattern in dairy goat testis
Author
Zheng, L;Zhu, H;Tang, F;Mu, H;Li, N;Wu, J;Hua, J;
Year
2015
Journal
Theriogenology
Application
IF and WB
PubMed ID: 25662202FoldedExpand
The distribution of α-kleisin during meiosis in the holocentromeric plant Luzula elegans
Author
Ma, W;Schubert, V;Martis, MM;Hause, G;Liu, Z;Shen, Y;Conrad, U;Shi, W;Scholz, U;Taudien, S;Cheng, Z;Houben, A;
Year
2016
Journal
Chromosome Res.
Application
WB
PubMed ID: 27294972FoldedExpand

If you couldn"t find the specific citation, you can:

Find All Citations
Submit a CitationGet a Publication Reward
Leave Us a Message

Recombinant Antibody Production Service

High-throughput Recombinant Antibody Production

Large-scale Antibody Production

Antibody Development Service

Rabbit MAb Production Service

Mouse MAb Production Service

Rabbit PAb Production Service

Phospho-Specific Antibody

Anti-Idiotype Antibody

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

More Antibody Production Service

Hybridoma Antibody Production

Chimeric Antibody

Antibody Fragment

Bispecific Antibody

We also have Immunoassay Service. Click to see more.

品牌介绍

北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。

公司介绍

品牌分类

Elisa试剂盒试剂蛋白质试剂抗体试剂 Gene Reagent

联络我们

服务热线：4000-520-616

（限工作日9:00-18:00）

QQ ：1570468124

手机：18915418616

官网：http://www.sinobiological.com/

Description

Related Products

Citations

Antibody Production Service

Anti-Histone H3 Antibody, Mouse Monoclonal General Information

Anti-Histone H3 Antibody, Mouse Monoclonal Validated Applications

Anti-Histone H3 Antibody, Mouse Monoclonal Images

Histone H3 Background Information

Citation List

If you couldn"t find the specific citation, you can:

Recombinant Antibody Production Service

Antibody Development Service

Fast Antibody Development Service

More Antibody Production Service