Anti-Transferrin Receptor/TFRC Antibody, Rabbit Polyclonal General Information
Anti-Transferrin Receptor/TFRC Antibody, Rabbit Polyclonal Validated Applications
| Application | Dilution |
|---|---|
| WB | 1:500-1:2000 |
| ELISA | 1:5000-1:10000 |
| IHC-P | 1:500-1:2000 |
| IP | 1-4 μL/mg of lysate |
Anti-Transferrin Receptor/TFRC Antibody, Rabbit Polyclonal Images
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Anti-TFRC rabbit polyclonal antibody at 1:500 dilution
Lane A: JurkatWhole Cell Lysate
Lysates/proteins at 30 μg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800)at 1/10000 dilution.
Developed using the Odyssey technique.
Performed under reducing conditions.
Predicted band size:98 kDa
Observed band size:80 kDa
-HB05JU0107-IP.png)
TFRC was immunoprecipitated using:
Lane A:0.5 mg Jurkat Whole Cell Lysate
Lane B:0.5 mg K562 Whole Cell Lysate
Lane C:0.5 mg 293T Whole Cell Lysate
Lane D:0.5 mg MCF-7 Whole Cell Lysate
2 µL anti-TFRC rabbit polyclonal antibody and 15 μl of50 %Protein G agarose.
Primary antibody:
Anti-TFRC rabbit polyclonal antibody,at 1:200 dilution
Secondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution
Developed using the odssey technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 100 kDa
Anti-Transferrin Receptor/TFRC Antibody, Rabbit Polyclonal: Synonyms
Transferrin Receptor/TFRC Background Information
- Douabin-GicquelV., et al., 2001,Hum. Genet. 109:393-401.
- Ryschich,E.et al., 2004,Eur J Cancer. 40(9):1418-22.
- TosoniD.,et al., 2005, Cell 123:875-888.
- WollscheidB., et al., 2009, Nat. Biotechnol. 27:378-386.
- Indications for distinct pathogenic mechanisms of asbestos and silica through gene expression profiling of the response of lung epithelial cellsAuthorPerkins, TN;Peeters, PM;Shukla, A;Arijs, I;Dragon, J;Wouters, EF;Reynaert, NL;Mossman, BT;Year2014JournalHum. Mol. Genet.ApplicationWBPubMed ID: 25351596FoldedExpand
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