中国生物/甲型流感H5N1（禽流感）血凝素/HA抗体，小鼠单克隆抗体/1/11048-MM01
商品编号：
11048-MM01
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Influenza A H5N1 (Avian Flu) Hemagglutinin / HA Antibody, Mouse MAb General Information
Product name
Influenza A H5N1 (Avian Flu) Hemagglutinin / HA Antibody, Mouse MAb
Validated applications
WB,ELISA,B/N
Application notes
IHC, FCM, IF, IP et al. applications haven"t been validated.(Antibody"s applications haven"t been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
H5N1 Hemagglutinin/HAHas cross-reactivity in ELISA withH5N1 (A/turkey/Turkey/1/2005) HAH5N1 (A/chicken/India/NIV33487/2006) HAH5N1 (A/Hong Kong/483/97) HAH5N1 (A/whooper swan/mongolia/244/2005) HAH5N1 (A/Viet nam/1194/2004) HAH5N1 (A/goose/Guiyang/337/2006) HAH5N1 (A/Indonesia/5/2005) HAH5N1 (A/Cambodia/R0405050/2007) HAH5N1 (A/duck/Hunan/795/2002) HAH5N1 (A/Egypt/2321-NAMRU3/2007) HAH5N1 (A/Egypt/N05056/2009) HAH5N3 (A/duck/Hokkaido/167/2007) HAH5N8 (A/duck/NY/191255-59/2002) HAH5N1 (A/bar-headed goose/Qinghai/14/2008) HAH5N1 (A/Common magpie/Hong Kong/2256/2006) HANo cross-reactivity in ELISA withH1N1 (A/California/04/2009) HAH1N1 (A/Brisbane/59/2007) HAH3N2 (A/Brisbane/10/2007) HAInfluenza B (B/Florida/4/2006) HAHuman cell lysate (293 cell line)
Immunogen
Recombinant H5N1 HA protein
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A virus H5N1 (Avian Flu) hemagglutinin extracellular domain. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #9F2E3F3
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Influenza A H5N1 (Avian Flu) Hemagglutinin / HA Antibody, Mouse MAb Validated Applications
| Application | Dilution |
|---|---|
| WB | 0.1-1 μg/mL |
| ELISA | 0.5-1 μg/mL |
Notes
| Block： | This antibody has neutralizing activity against H5N1 (A/Anhui/1/2005) strain and H5N1 (A/bar-headed/goose/Qinghai/14/2008) strain, but no neutralizing activity against H1N1 strains through pseudotyped neutralization assay. Other H5N1 strains were not tested. The concentration of this antibody with 50% neutralization is 2 μg/ml. |
Please Note: Optimal concentrations/dilutions should be determined by the end user.
Hemagglutinin/HA Background Information
The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells" sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
References
- White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
- Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
- Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
- Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018
- Hemagglutinin stalk domain from H5N1 strain as a potentially universal antigen.AuthorK Uranowska, J Tyborowska, A Jurek…Year2013JournalActa biochimica polonicaPubMed ID: 25210720FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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