Sino Biological/PTEN cDNA开放阅读框克隆，人，未标记/1/HG10421-UT
商品编号：
HG10421-UT
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PTEN cDNA ORF Clone, Human, untagged: General Information
Gene
Species
Human
NCBI Ref Seq
NM_000314.4
RefSeq ORF Size
1212 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human phosphatase and tensin homolog (PTEN).
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-untagged
Restriction Sites
KpnI + XbaI (6.1kb + 1.21kb)
Sequencing Primers
T7( 5" TAATACGACTCACTATAGGG 3" )BGH( 5" TAGAAGGCACAGTCGAGG 3" )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Ampicillin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
PTEN cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
PTEN cDNA ORF Clone, Human, untagged: Synonyms
10q23del cDNA ORF Clone, Human; BZS cDNA ORF Clone, Human; CWS1 cDNA ORF Clone, Human; DEC cDNA ORF Clone, Human; GLM2 cDNA ORF Clone, Human; MHAM cDNA ORF Clone, Human; MMAC1 cDNA ORF Clone, Human; PTEN1 cDNA ORF Clone, Human; TEP1 cDNA ORF Clone, Human
PTEN Background Information
PTEN gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translations start codons results in multiple transcript variants encoding different isoforms.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
phosphatase and tensin homolog
Research Areas
- Phosphatases and Regulators
- Tumor Suppressors
- Cancer Drug Targets
Related Pathways
p53 Pathway
VEGF Signaling Pathway
AKT Signaling Pathway
B Cell Receptor Signaling Pathway
References
- Shinde SR, Maddika S. PTEN modulates EGFR late endocytic trafficking and degradation by dephosphorylating Rab7. Nature Communications. 2016;7:10689.
PTEN cDNA ORF Clone, Human,Related Products
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- PTEN cDNA ORF Clone, Human, N-His tagHG10421-NH
- PTEN cDNA ORF Clone, Human, N-Myc tagHG10421-NM
- PTEN cDNA ORF Clone, Human, N-HA tagHG10421-NY
- PTEN cDNA ORF Clone, Human, C-His tagHG10421-CH
- PTEN cDNA ORF Clone, Human, C-Myc tagHG10421-CM
- PTEN cDNA ORF Clone, Human, N-GFPSpark® tagHG10421-ANG
- PTEN cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tagHG10421-CF
- PTEN cDNA ORF Clone, Human, C-GFPSpark® tagHG10421-ACG
- PTEN cDNA ORF Clone, Human, C-HA tagHG10421-CY
- PTEN cDNA ORF Clone in Cloning Vector, HumanHG10421-M
- PTEN Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tagHG10421-ACGLN
- PTEN cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tagHG10421-NF
- Human PTEN qPCR Primer PairHP100451
- BTG2 is a tumor suppressor gene upregulated by p53 and PTEN in human bladder carcinoma cellsAuthorTsui, KH;Chiang, KC;Lin, YH;Chang, KS;Feng, TH;Juang, HH;Year2017JournalCancer MedApplicationexpressionPubMed ID: 29239139FoldedExpand
- PTEN-deficient cells prefer glutamine for metabolic synthesisAuthorWang, Z;Chen, D;Piao, H;Hua, X;Year2020JournalActa Biochimica et Biophysica SinicaApplicationtransfectionFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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