Sino Biological/PPARGC1A/PGC1 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag/1/HG12107-CF
商品编号：
HG12107-CF
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PPARGC1A/PGC1 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: General Information
Gene
Species
Human
NCBI Ref Seq
NM_013261.3
RefSeq ORF Size
2397 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1182 A>G and 1584 G>A not causing the amino acid variation.
Description
Full length Clone DNA of Human peroxisome proliferator-activated receptor gamma, coactivator 1 alpha with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Restriction Sites
KpnI + NotI (6kb + 2.44kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5" TAATACGACTCACTATAGGG 3" )BGH( 5" TAGAAGGCACAGTCGAGG 3" )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
PPARGC1A/PGC1 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
PPARGC1A/PGC1 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: Validated Images
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
PPARGC1A/PGC1 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: Synonyms
LEM6 cDNA ORF Clone, Human; PGC-1(alpha) cDNA ORF Clone, Human; PGC-1v cDNA ORF Clone, Human; PGC1 cDNA ORF Clone, Human; PGC1A cDNA ORF Clone, Human; PPARGC1 cDNA ORF Clone, Human
PPARGC1A/PGC1 Background Information
Peroxisome proliferator-activated receptor γ coactivator 1α (PGC1α) encoded by the PPARGC1A gene is a vital regulator of glucose and fatty acid oxidation, mitochondrial biogenesis, and skeletal muscle fiber conversion. PGC-1α is a transcriptional co-activator that has been implicated in the pathogenesis of neurodegenerative disorders. As a central regulator of mitochondrial biogenesis and metabolism, PPARGC1A acts as a powerful coactivator of many transcriptional factors that relate to steroidogenesis. The mitochondrial regulator PPARGC1A regulates mitochondrial biogenesis, reducing senescence of vascular smooth muscle cells (VSMCs). PPARGC1A upregulates autophagy reducing senescence by an SQSTM1-dependent mechanism. Evaluation in human normal lung and metastatic lung cancer cell lines revealed that the expression of PPARGC1A was upregulated in metastatic lung cancer cell lines.
Full Name
peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Related Pathways
AMPK Signaling Pathway
MAPK-Erk Pathway
References
- Zhang GM, et al. (2019) Effect of ppargc1a on the development and metabolism of early rabbit embryos in vitro. Mol Reprod Dev 86 (11): 1758-1770.
- Salazar G, et al. (2020) Sqstm1/p62 and ppargc1a/pgc-1alpha at the interface of autophagy and vascular senescence. Autophagy 16 (6): 1092-1110.
- Li JD, et al. (2017) Ppargc1a is upregulated and facilitates lung cancer metastasis. Exp Cell Res 359 (2): 356-360.
- Chen Y, et al. (2019) Meta-analyses of the association between the ppargc1a gly482ser polymorphism and athletic performance. Biol Sport 36 (4): 301-309.
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- N6-methyladenosine demethylase FTO suppresses clear cell renal cell carcinoma through a novel FTO-PGC-1α signalling axisAuthorZhuang, C;Zhuang, C;Luo, X;Huang, X;Yao, L;Li, J;Li, Y;Xiong, T;Ye, J;Zhang, F;Gui, Y;Year2019JournalJ. Cell. Mol. Med.PubMed ID: 30648791FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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