Sino Biological/PADI4 / PAD4 cDNA ORF Clone, Human, N-His tag/1/HG11072-NH
商品编号：
HG11072-NH
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PADI4 / PAD4 cDNA ORF Clone, Human, N-His tag: General Information
Gene
Species
Human
NCBI Ref Seq
NM_012387.2
RefSeq ORF Size
2037 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 394T/C not causing the amino acid variation.
Description
Full length Clone DNA of Human peptidyl arginine deiminase, type I V with N terminal His tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-N-His
Restriction Sites
KpnI + XbaI(6kb+2.04kb)
Tag Sequence
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequencing Primers
T7( 5" TAATACGACTCACTATAGGG 3" )BGH( 5" TAGAAGGCACAGTCGAGG 3" )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
PADI4 / PAD4 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
PADI4 / PAD4 cDNA ORF Clone, Human, N-His tag: Validated Images
PADI4 / PAD4 cDNA ORF Clone, Human, N-His tag: Synonyms
PAD cDNA ORF Clone, Human; PAD4 cDNA ORF Clone, Human; PADI5 cDNA ORF Clone, Human; PDI4 cDNA ORF Clone, Human; PDI5 cDNA ORF Clone, Human
PADI4 / PAD4 Background Information
Protein-arginine deiminase type-4, also known as HL-6 PAD, Peptidylarginine deiminase IV, Protein-arginine deiminase type I V and PADI4, is a cytoplasm and nucleus protein that belongs to the protein arginine deiminase family. PADI4 is expressed in CD34+ stem cells in normal tissues, and many more CD34+ cells expressing PADI4 are present in tumour tissues. PADI4 post-translationally converts peptidylarginine to citrulline, a process called citrullination. Studies have demonstrated the high expression of PADI4 in various malignant tumor tissues. PADI4 is also expressed at high levels in the blood of patients with some malignant tumors. Citrullination of histone, cytokeratin, antithrombin and fibronectin have been confirmed to be involved in abnormal apoptosis, high coagulation, and disordered cell proliferation and differentiation, all of which are main features of malignant tumors. PADI4 may play an important role in tumorigenesis. Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
Full Name
peptidyl arginine deiminase, type IV
References
- NakashimaK. et al.,1999, J. Biol. Chem. 274: 27786-92.
- SuzukiA. et al., 2003, Nat. Genet. 34:395-402.
- Nakayama-HamadaM. et al., 2005, Biochem. Biophys. Res. Commun. 327:192-200.
- Chang,X.et al., 2010, Cancer Cell Int10:7.
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- NETosis proceeds by cytoskeleton and endomembrane disassembly and PAD4-mediated chromatin de-condensation and nuclear envelope ruptureAuthorWaterman, CM;Thiam, HR;Wong, SL;Qiu, R;Year2019JournalBioRxivApplicationtemplatePubMed ID: 31214999FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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