Sino Biological/EGFR cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag/1/MG51091-CF
商品编号：
MG51091-CF
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EGFR cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: General Information
Gene
Species
Mouse
NCBI Ref Seq
NM_207655.2
RefSeq ORF Size
3633 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 40 T>C, 1725 A>G and 2799 G>A not causing the amino acid variation.
Description
Full length Clone DNA of Mouse epidermal growth factor receptor with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Restriction Sites
KpnI + NotI (6kb + 3.68kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5" TAATACGACTCACTATAGGG 3" )BGH( 5" TAGAAGGCACAGTCGAGG 3" )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
EGFR cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
EGFR cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: Validated Images
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
EGFR cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: Synonyms
9030024J15Rik cDNA ORF Clone, Mouse; AI552599 cDNA ORF Clone, Mouse; Erbb cDNA ORF Clone, Mouse; Errb1 cDNA ORF Clone, Mouse; Errp cDNA ORF Clone, Mouse; wa-2 cDNA ORF Clone, Mouse; wa2 cDNA ORF Clone, Mouse; Wa5 cDNA ORF Clone, Mouse
EGFR Background Information
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
epidermal growth factor receptor
Research Areas
- Cancer Drug Targets
- Receptor Tyrosine Kinases (RTKs)
Related Pathways
EGFR Signaling Pathway
Jak-Stat Signaling Pathway
References
- Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
- Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
- Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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- TFF3 is a ligand for LINGO2 that de-represses EGFR to control disease outcome during colitis and gastrointestinal nematode infectionAuthorJi, Y;Wei, Y;Park, J;Hung, L;Young, T;Herbine, K;Oniskey, T;Pastore, C;Nieves, W;Somsouk, M;Herbert, D;Year2018JournalbioRxivFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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