Sino Biological/Influenza A H10N8 (A/duck/Guangdong/E1/2012) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized)/1/VG40351-UT-免疫在线蚂蚁淘旗下平台

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商品详细Sino Biological/Influenza A H10N8 (A/duck/Guangdong/E1/2012) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized)/1/VG40351-UT

Sino Biological/Influenza A H10N8 (A/duck/Guangdong/E1/2012) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized)/1/VG40351-UT

商品编号： VG40351-UT

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Influenza A H10N8 (A/duck/Guangdong/E1/2012) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized): General Information

Gene

Species

H10N8

RefSeq ORF Size

1686 bp

Sequence Description

A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with AFJ19053.1.

Description

Full length Clone DNA of Influenza A H10N8 (A/duck/Guangdong/E1/2012) Hemagglutinin.

Plasmid

Promoter

Enhanced CMV promoter

Vector

pCMV3-untagged

Restriction Sites

KpnI + XbaI (6.1kb + 1.69kb)

Sequencing Primers

T7( 5" TAATACGACTCACTATAGGG 3" )BGH( 5" TAGAAGGCACAGTCGAGG 3" )

Quality Control

The plasmid is confirmed by full-length sequencing.

Screening

Antibiotic in E.coli

Ampicillin

Antibiotic in Mammalian cell

Hygromycin

Application

Stable or Transient mammalian expression

Storage & Shipping

Shipping

Each tube contains lyophilized plasmid.

Storage

The lyophilized plasmid can be stored at ambient temperature for three months.

Hemagglutinin/HA cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Influenza A H10N8 (A/duck/Guangdong/E1/2012) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized): Validated Images

other Green fluorescent protein / GFP Gene Plasmid Map 5613

Hemagglutinin/HA Background Information

The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells" sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

Full Name

Harvey rat sarcoma viral oncogene homolog

References

White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018

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Influenza A H11N9 (A/mallard/Alberta/294/1977) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, N-His tagVG11704-NH
Influenza A H11N9 (A/mallard/Alberta/294/1977) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-His tagVG11704-CH

Hemagglutinin/HA cDNA ORF Clone, Influenza B,Related Products

Influenza B (B/Hong Kong/05/1972) Hemagglutinin / HA Gene Lentiviral ORF cDNA expression plasmid, C-GFPSpark tag(Codon Optimized)VG40461-ACGLN
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Influenza B (B/Utah/02/2012) Hemagglutinin / HA Gene Lentiviral ORF cDNA expression plasmid, C-GFPSpark tag(Codon Optimized)VG40463-ACGLN
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Influenza B (B/Malaysia/2506/2004) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-OFPSpark / RFP tagVG11716-ACR
Influenza B (B/Victoria/504/2000) Hemagglutinin / HA (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-OFPSpark / RFP tagVG40391-ACR
Influenza B (B/Victoria/504/2000) Hemagglutinin / HA (Codon Optimized) Gene ORF cDNA clone expression plasmid, N-Flag tagVG40391-NF
Influenza B (B/Wisconsin/01/2010) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized)VG40526-UT
Influenza B (Brisbane/60/2008) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized), N-Myc-taggedVG40016-NM
Influenza B (B/Brisbane/60/2008) Hemagglutinin / HA (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-OFPSpark / RFP tagVG40016-ACR
Influenza B (B/Bangladesh/5945/2009) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized)VG40503-G-N
Influenza B (B/Victoria/527/2005) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized)VG40517-G-N

Hemagglutinin/HA cDNA ORF Clone, H6N6,Related Products

Influenza A H6N6 (A/duck/Eastern China/11/2009) Hemagglutinin / HA Gene Lentiviral ORF cDNA expression plasmid, C-GFPSpark tag(Codon Optimized)VG40399-ACGLN
Influenza A H6N6 (A/duck/Eastern China/11/2009) Hemagglutinin / HA (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-His tagVG40399-CH
Influenza A H6N6 (A/duck/Eastern China/11/2009) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized)VG40399-G

Hemagglutinin/HA cDNA ORF Clone, H1N3,Related Products

Influenza A H1N3 (A/duck/NZL/160/1976) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-Myc tagVG11685-CM

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Influenza A H11N2 (A/duck/Yangzhou/906/2002) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, N-Flag tagVG11705-NF
Influenza A H11N2 (A/duck/Yangzhou/906/2002) Hemagglutinin Gene ORF cDNA clone expression plasmid (Codon Optimized), N-Myc-taggedVG11705-NM
Influenza A H11N2 (A/duck/Yangzhou/906/2002) Hemagglutinin Gene ORF cDNA clone expression plasmid (Codon Optimized), C-GFPSpark-taggedVG11705-ACG
Influenza A H11N2 (A/duck/Yangzhou/906/2002) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-HA tagVG11705-CY

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Influenza A H1N2 (A/swine/Guangxi/13/2006) Hemagglutinin Gene ORF cDNA clone expression plasmid (Codon Optimized)VG11703-UT

Hemagglutinin/HA cDNA ORF Clone, H13N8,Related Products

Influenza A H13N8 (A/black-headed gull/Netherlands/1/00) Hemagglutinin Gene ORF cDNA clone expression plasmid (Codon Optimized), N-Myc-taggedVG11721-NM
Influenza A H13N8 (A/black-headed gull/Netherlands/1/00) Hemagglutinin Gene ORF cDNA clone expression plasmid (Codon Optimized)VG11721-UT
Influenza A H13N8 (A/black-headed gull/Netherlands/1/00) Hemagglutinin Gene ORF cDNA clone expression plasmid (Codon Optimized), C-Flag tagVG11721-CF

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Influenza A H15N8 (A/duck/AUS/341/1983) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-OFPSpark / RFP tagVG11720-ACR
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Influenza A H15N8 (A/duck/AUS/341/1983) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, N-HA tagVG11720-NY

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Influenza A H9N2 (A/Guinea fowl/Hong Kong/WF10/99) Hemagglutinin Gene ORF cDNA clone expression plasmid (Codon Optimized), C-Flag tagVG11719-CF
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Influenza A H9N2 (A/Guinea fowl/Hong Kong/WF10/99) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, N-His tagVG11719-NH
Influenza A H9N2 (A/Chicken/Hong Kong/G9/97) Hemagglutinin Gene ORF cDNA clone expression plasmidVG40036-UT

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Influenza A H6N1 (A/quail/Hong Kong/1721-30/99) Hemagglutinin / HA (Codon Optimized) Gene ORF cDNA clone expression plasmid, N-Flag tagVG40397-NF

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Influenza A H8N4 (A/pintail duck/Alberta/114/1979) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, N-His tagVG11722-NH

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Influenza A H6N2 (A/chicken/Guangdong/C273/2011) Hemagglutinin / HA (Codon Optimized) Gene ORF cDNA clone expression plasmid, N-Myc tagVG40398-NM
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Influenza A H5N2 (A/American green-winged teal/California/HKWF609/2007) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-OFPSpark / RFP tagVG11699-ACR
Influenza A H5N2 (A/American green-winged teal/California/HKWF609/07) Hemagglutinin Gene ORF cDNA clone expression plasmid (Codon Optimized), C-Flag tagVG11699-CF

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Influenza A H12N5 (A/green-winged teal/ALB/199/1991 ) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, N-HA tagVG11718-NY
Influenza A H12N5 (A/green-winged teal/ALB/199/1991 ) Hemagglutinin Gene ORF cDNA clone expression plasmid (Codon Optimized)VG11718-UT
Influenza A H12N5 (A/green-winged teal/ALB/199/1991 ) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-OFPSpark / RFP tagVG11718-ACR
Influenza A H12N5 (A/green-winged teal/ALB/199/1991 ) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-Myc tagVG11718-CM

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Influenza A H4N6 (A/mallard/Ohio/657/2002) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-His tagVG11714-CH
Influenza A H4N6 (A/mallard/Ohio/657/2002) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-HA tagVG11714-CY
Influenza A H4N6 (A/mallard/Ohio/657/2002) Hemagglutinin Gene ORF cDNA clone expression plasmid (Codon Optimized)VG11714-UT
Influenza A H4N6 (A/Swine/Ontario/01911-1/99) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, N-His tagVG11706-NH
Influenza A H4N6 (A/Swine/Ontario/01911-1/99) Hemagglutinin Gene ORF cDNA clone expression plasmid (Codon Optimized)VG11706-UT

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Influenza A H4N8 (chicken/Alabama/1/1975) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized), N-Myc-taggedVG40025-NM
Influenza A H4N8 (chicken/Alabama/1/1975) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized)VG40025-UT

Hemagglutinin/HA cDNA ORF Clone, H7N3,Related Products

Influenza A H7N3 (A/chicken/SK/HR-00011/2007) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized)VG40129-UT

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Influenza A H2N2 (A/Canada/720/2005) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, N-HA tagVG11688-NY
Influenza A H2N2 (A/Canada/720/2005) Hemagglutinin (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-Myc tagVG11688-CM

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Influenza A H4N2 (A/duck/Hunan/8-19/2009) Hemagglutinin / HA (Codon Optimized) Gene ORF cDNA clone expression plasmid, N-His tagVG40390-NH
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Influenza A H4N2 (A/duck/Hunan/8-19/2009) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized)VG40390-G

Hemagglutinin/HA cDNA ORF Clone, H16N3,Related Products

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Influenza A H16N3 (A/black-headed gull/Sweden/5/99) Hemagglutinin Gene ORF cDNA clone expression plasmid (Codon Optimized), C-Flag tagVG11711-CF

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Influenza A H4N4 (mallard duck/Alberta/299/1977) Hemagglutinin / HA Gene ORF cDNA clone expression plasmid (Codon Optimized), N-Myc-taggedVG40008-NM
Influenza A H4N4 (mallard duck/Alberta/299/1977) Hemagglutinin / HA (Codon Optimized) Gene ORF cDNA clone expression plasmid, C-His tagVG40008-CH

Double-layered protein nanoparticles induce broad protection against divergent influenza A viruses
Author
Deng, L;Mohan, T;Chang, TZ;Gonzalez, GX;Wang, Y;Kwon, YM;Kang, SM;Compans, RW;Champion, JA;Wang, BZ;
Year
2018
Journal
Nat Commun
Application
expression
PubMed ID: 29367723FoldedExpand

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品牌介绍

北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。

公司介绍

品牌分类

Elisa试剂盒试剂蛋白质试剂抗体试剂 Gene Reagent

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