Sino Biological/MDM2 cDNA开放阅读框克隆，人，N-DYKDDDDK（Flag®；）标签/1/HG11206-NF
商品编号：
HG11206-NF
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
MDM2 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: General Information
Gene
Species
Human
NCBI Ref Seq
NM_002392.3
RefSeq ORF Size
1494 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human MDM2 oncogene, E3 ubiquitin protein ligase with N terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-N-FLAG
Restriction Sites
KpnI + NotI (6kb + 1.54kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5" TAATACGACTCACTATAGGG 3" )BGH( 5" TAGAAGGCACAGTCGAGG 3" )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
MDM2 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
MDM2 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: Validated Images
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
MDM2 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: Synonyms
ACTFS cDNA ORF Clone, Human; hdm2 cDNA ORF Clone, Human; HDMX cDNA ORF Clone, Human
MDM2 Background Information
MDM2 (MDM2 Proto-Oncogene) is a Protein Coding gene. 11 alternatively spliced human isoforms have been reported. MDM2 gene encodes a nuclear-localized E3 ubiquitin ligase, which belongs to the MDM2/MDM4 family. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. It Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Overexpression or amplification of this locus is detected in a variety of different cancers. MDM2 is widely expressed in the colon, bone marrow, and other tissues. Diseases associated with MDM2 include Lessel-Kubisch Syndrome and Accelerated Tumor Formation.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
MDM2 proto-oncogene, E3 ubiquitin protein ligase
Research Areas
- Cancer Drug Targets
Related Pathways
EGFR Signaling Pathway
p53 Pathway
AKT Signaling Pathway
References
- Zhuo X, Ye H, Li Q, Xiang Z, Zhang X. Is MDM2 SNP309 Variation a Risk Factor for Head and Neck Carcinoma?: An Updated Meta-Analysis Based on 11,552 Individuals. Langevin. S, ed. Medicine. 2016;95(9):e2948.
MDM2 cDNA ORF Clone, Human,Related Products
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- MDM2 cDNA ORF Clone, Human, C-HA tagHG11206-CY
- MDM2 cDNA ORF Clone in Cloning Vector, HumanHG11206-M
- MDM2 cDNA ORF Clone, Human, N-Myc tagHG11206-NM
- MDM2 cDNA ORF Clone, Human, C-GFPSpark® tagHG11206-ACG
- MDM2 cDNA ORF Clone, Human, C-His tagHG11206-CH
- MDM2 cDNA ORF Clone, Human, untaggedHG11206-UT
- MDM2 cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tagHG11206-CF
- MDM2 cDNA ORF Clone, Human, N-GFPSpark® tagHG11206-ANG
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- MDM2 cDNA ORF Clone, Human, N-HA tagHG11206-NY
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- Human MDM2 qPCR Primer PairHP101108
MDM2 cDNA ORF Clone, Mouse,Related Products
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- MDM2 cDNA ORF Clone, Mouse, C-Myc tagMG50533-CM
- MDM2 cDNA ORF Clone, Mouse, N-GFPSpark® tagMG50533-ANG
- MDM2 cDNA ORF Clone, Mouse, N-OFPSpark® tagMG50533-ANR
- MDM2 cDNA ORF Clone, Mouse, C-His tagMG50533-CH
- MDM2 cDNA ORF Clone, Mouse, N-Myc tagMG50533-NM
- MDM2 cDNA ORF Clone in Cloning Vector, MouseMG50533-M
- MDM2 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tagMG50533-ACGLN
- MDM2 cDNA ORF Clone, Mouse, N-DYKDDDDK (Flag®) tagMG50533-NF
- MDM2 cDNA ORF Clone, Mouse, untaggedMG50533-UT
- MDM2 cDNA ORF Clone, Mouse, C-Myc tagMG50533-CY
- MDM2 cDNA ORF Clone, Mouse, C-OFPSpark® tagMG50533-ACR
- Mouse MDM2 qPCR Primer PairMP200525
- A DHX9-lncRNA-MDM2 interaction regulates cell invasion and angiogenesis of cervical cancerAuthorDing, X;Jia, X;Wang, C;Xu, J;Gao, SJ;Lu, C;Year2018JournalCell Death Differ.PubMed ID: 30518908FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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