Sino Biological/HIF-1 alpha cDNA ORF Clone in Cloning Vector, Human/1/HG11977-G
商品编号：
HG11977-G
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HIF-1 alpha cDNA ORF Clone in Cloning Vector, Human: General Information
Gene
Species
Human
NCBI Ref Seq
NM_001530
RefSeq ORF Size
2481 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor).
Plasmid
Vector
pGEM-T Vector
Sequencing Primers
SP6 and T7 or M13-47 and RV-M
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Ampicillin
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
HIF-1 alpha cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
HIF-1 alpha cDNA ORF Clone in Cloning Vector, Human: Synonyms
bHLHe78 cDNA ORF Clone, Human; HIF-1A cDNA ORF Clone, Human; HIF-1alpha cDNA ORF Clone, Human; HIF1 cDNA ORF Clone, Human; HIF1-ALPHA cDNA ORF Clone, Human; MOP1 cDNA ORF Clone, Human; PASD8 cDNA ORF Clone, Human
HIF-1 alpha Background Information
HIF-1 alpha, also known as HIF1A, contains 1 basic helix-loop-helix (bHLH) domain, 1 PAC (PAS-associated C-terminal) domain, and 2 PAS (PER-ARNT-SIM) domains. It is one of the two subunits of Hypoxia-inducible factor-1 (HIF1). HIF1 is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). HIF-1 alpha is expressed in most tissues with the highest levels in the kidney and heart. It is overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. HIF-1 alpha functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, it activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1A plays an essential role in embryonic vascularization, tumor angiogenesis, and the pathophysiology of ischemic disease. HIF-1 alpha binds to core DNA sequence 5"-[AG]CGTG-3" within the hypoxia response element (HRE) of target gene promoters. Activation requires the recruitment of transcriptional coactivators such as CREBPB and EP300.
Full Name
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Research Areas
- Cancer Drug Targets
Related Pathways
p53 Pathway
References
- Zhou Q, et al. (2011) Loss of either hypoxia inducible factor 1 or 2 promotes lung cancer cell colonization. Cell Cycle. 10(13):2233-4.
- Krishnan J, et al. (2012) Dietary obesity-associated Hif1 alpha activation in adipocytes restricts fatty acid oxidation and energy expenditure via suppression of the Sirt2-NAD+ system. Genes Dev. 26(3):259-70.
- Novo E, et al. (2012) The biphasic nature of hypoxia-induced directional migration of activated human hepatic stellate cells. J Pathol. 226(4):588-97.
- Dungwa JV, et al. (2011) Overexpression of carbonic anhydrase and HIF-1 in Wilms tumours. BMC Cancer. 11:390.
HIF-1 alpha cDNA ORF Clone, Human,Related Products
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- HIF-1 alpha cDNA ORF Clone, Human, untaggedHG11977-UT
- HIF-1 alpha cDNA ORF Clone, Human, C-GFPSpark® tagHG11977-ACG
- HIF-1 alpha cDNA ORF Clone, Human, C-His tagHG11977-CH
- HIF-1 alpha cDNA ORF Clone, Human, C-OFPSpark® tagHG11977-ACR
- HIF-1 alpha Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tagHG11977-ACGLN
- HIF-1 alpha cDNA ORF Clone, Human, N-Myc tagHG11977-NM
- HIF-1 alpha cDNA ORF Clone, Human, N-GFPSpark® tagHG11977-ANG
- HIF-1 alpha cDNA ORF Clone, Human, N-HA tagHG11977-NY
- HIF-1 alpha cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tagHG11977-NF
- HIF-1 alpha cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tagHG11977-CF
- HIF-1 alpha cDNA ORF Clone, Human, C-HA tagHG11977-CY
- HIF-1 alpha cDNA ORF Clone, Human, N-His tagHG11977-NH
- HIF-1 alpha cDNA ORF Clone, Human, N-OFPSpark® tagHG11977-ANR
- Human HIF-1 alpha qPCR Primer PairHP101522
- ADAM17 mediates hypoxia-induced drug resistance in hepatocellular carcinoma cells through activation of EGFR/PI3K/Akt pathwayAuthorWang, XJ;Feng, CW;Li, M;Year2013JournalMol. Cell. Biochem.Applicationcloning and transfectionPubMed ID: 23625205 FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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