Sino Biological/NGF ELISA试剂盒，人/1/KIT11050
商品编号：
KIT11050
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
NGF ELISA Kit, Human: General Information
Product name
NGF ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Cell supernatant
Sensitivity
1.81 pg/mL
Assay range
3.13-200 pg/mL
Specificity
Recognizes both recombinant and natural Human NGF
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human β-NGF control were assayed for interference. No significant interference was observed. High significant interference were observed within standard curve of recombinant mouse β-NGF.
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 41.41 | 72.96 | 138.46 |
| SD | 0.89 | 1.44 | 4.37 |
| CV(%) | 2.1% | 2.0% | 3.2% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 38.31 | 68.41 | 118.19 |
| SD | 1.57 | 2.37 | 4.52 |
| CV(%) | 4.1% | 3.5% | 3.8% |
Recovery
The recovery of Human NGF spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| supernatant (n=3) |
| Average % Recovery |
| 83 |
| Range |
| 73-89% |
Linearity
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 110% |
| 1:4 | 115% |
| 1:8 | 105% |
| 1:16 | 86% |
Materials provided
1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution
Product overview
This NGF ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human NGF protein in Cell supernatant . It contains recombinant Human NGF, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA
NGF ELISA Kit, Human: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human β-NGF. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. High cross-reactivity were observed within standard curve of recombinant mouse β-NGF.
NGF ELISA Kit, Human: Synonyms
Beta-NGF ELISA Kit, Human; HSAN5 ELISA Kit, Human; NGFB ELISA Kit, Human
NGF Background Information
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth-stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein that homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. Also, previous studies indicated that NGF may also have an important role in the regulation of the immune system.
Full Name
nerve growth factor (beta polypeptide)
Related Pathways
Death Receptor Signaling
References
- Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72.
- Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson"s disease. J Mol Neurosci. 35(2): 161-9.
- Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.
- Nerve Growth Factor Gene Delivery across the Blood–Brain Barrier to Reduce Beta Amyloid Accumulation in AD MiceAuthorRodrigues, B;Kanekiyo, T;Singh, J;Year2020JournalMol. PharmaceuticsApplicationElisaFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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