Sino Biological/CD200 ELISA Kit, Human/1/KIT10886
商品编号：
KIT10886
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
CD200 ELISA Kit, Human: General Information
Product name
CD200 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Recombinant protein
Sensitivity
30.3 pg/mL
Assay range
62.5-4000 pg/mL
Specificity
Recognizes both recombinant and natural Human CD200
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 283.00 | 602.00 | 966.00 |
| SD | 10.11 | 30.02 | 50.01 |
| CV(%) | 2.6% | 2.9% | 3.1% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 254.00 | 582.00 | 943.00 |
| SD | 20.55 | 20.36 | 90.68 |
| CV(%) | 6.7% | 3.2% | 9.7% |
Recovery
The recovery of Human CD200 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
| Sample |
| supernatant (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
| Sample |
| urine (n=3) |
| Average % Recovery |
| 84 |
| Range |
| 81 -87% |
Linearity
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 93% |
| 1:4 | 98% |
| 1:8 | 109% |
| 1:16 | 95% |
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 93% |
| 1:4 | 98% |
| 1:8 | 109% |
| 1:16 | 95% |
Materials provided
1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution
Product overview
This CD200 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD200 protein in Recombinant protein . It contains recombinant Human CD200, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA
CD200 ELISA Kit, Human: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant Human CD200. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CD200 ELISA Kit, Human: Synonyms
MOX1 ELISA Kit, Human; MOX2 ELISA Kit, Human; MRC ELISA Kit, Human; OX-2 ELISA Kit, Human; OX2 ELISA Kit, Human
CD200 Background Information
CD200 (OX-2) is a cell surface glycoprotein that imparts immune privileges by suppressing alloimmune and autoimmune responses through its receptor, CD200R, expressed primarily on myeloid cells. Signals delivered through the CD200:CD200R axis have been shown to play an important role in the regulation of anti-tumor immunity, and overexpression of CD200 has been reported in a number of malignancies, including CLL, as well as on cancer stem cells. The role of CD200-CD200R signaling in immune regulation of the central nervous system has become a popular field of research in recent years. Many studies have shown that there is a close correlation between CD200-CD200R, microglia activation, and Parkinson"s disease (PD). The ability of CD200 to suppress myeloid cell activation is critical for maintaining normal tissue homeostasis but may also enhance the survival of migratory neoplastic cells. CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 has been characterized as an important immunoregulatory molecule, increased expression of which can lead to decreased transplant rejection, autoimmunity, and allergic disease. Elevated CD200 expression has been reported to be associated with poor prognosis in some human malignancies. Besides, CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
Cd200 molecule
Research Areas
- Neuroinflammation
- Cancer Drug Targets
References
- Minas K, et al. (2006) Is the CD200/CD200 receptor interaction more than just a myeloid cell inhibitory signal? Crit Rev Immunol. 26(3): 213-30.
- Wang XJ, et al. (2007) CD200-CD200R regulation of microglia activation in the pathogenesis of Parkinson"s disease. J Neuroimmune Pharmacol. 2(3): 259-64.
- Wong KK, et al. (2010) The role of CD200 in immunity to B cell lymphoma. J Leukoc Biol. 88(2): 361-72.
- Estrogen-regulated CD200 inhibits macrophage phagocytosis in endometriosisAuthorWeng, L;Hou, S;Lei, S;Peng, H;Li, M;Zhao, D;Year2020JournalJournal of Reproductive ImmunologyApplicationELISAFoldedExpand
- Reduced Serum Levels of Cluster of Differentiation 200 in Alcohol-Dependent PatientsAuthorChaturvedi, A;Rao, G;Praharaj, SK;Guruprasad, KP;Pais, V;Year2020JournalAlcohol Alcohol.ApplicationElisaPubMed ID: 32363396FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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