Sino Biological/L1CAM ELISA Kit, Human/1/KIT10140
商品编号：
KIT10140
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
L1CAM ELISA Kit, Human: General Information
Product name
L1CAM ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
8.31 pg/mL
Assay range
39.06-2500 pg/mL
Specificity
Recognizes both recombinant and natural Human L1CAM
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 383.50 | 726.69 | 1,447.90 |
| SD | 20.08 | 41.19 | 48.79 |
| CV(%) | 5.2% | 5.7% | 3.4% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 393.77 | 792.05 | 1,524.00 |
| SD | 17.77 | 74.35 | 109.17 |
| CV(%) | 4.5% | 9.4% | 7.2% |
Recovery
The recovery of Human L1CAM spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 91 |
| Range |
| 81-108% |
Linearity
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 94% |
| 1:4 | 100% |
| 1:8 | 100% |
| 1:16 | 110% |
Materials provided
1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution
Product overview
This L1CAM ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human L1CAM protein in Serum . It contains recombinant Human L1CAM, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA
L1CAM ELISA Kit, Human: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD171. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
L1CAM ELISA Kit, Human: Synonyms
CAML1 ELISA Kit, Human; CD171 ELISA Kit, Human; HSAS ELISA Kit, Human; HSAS1 ELISA Kit, Human; MASA ELISA Kit, Human; MIC5 ELISA Kit, Human; N-CAM-L1 ELISA Kit, Human; N-CAML1 ELISA Kit, Human; NCAM-L1 ELISA Kit, Human; S10 ELISA Kit, Human; SPG1 ELISA Kit, Human
L1CAM Background Information
L1 cell adhesion molecule (L1CAM), also designated as CD171, is a cell adhesion receptor of the immunoglobulin superfamily, known for its roles in nerve cell function. While originally believed to be present only in brain cells, in recent years L1-CAM has been detected in other tissues, and a variety of cancer cells, including some common types of human cancer. L1CAM interacts with a variety of ligands including axonin-1, CD9, neurocan, and integrins, and it has been revealed that the RGD motif in the sixth Ig domain of L1CAM is a binding site for integrins, thus important for nuclear signaling. Disruption of L1CAM function causes three X-linked neurological syndromes, i.e. hydrocephalus, MASA syndrome (mental retardation, aphasia, shuffling gait, and adducted thumbs), and spastic paraplegia syndrome. Overexpression of L1CAM in normal and cancer cells increased motility, enhanced growth rate, and promoted cell transformation and tumorigenicity. Recent work has identified L1CAM (CD171) as a novel marker for human carcinoma progression, and a candidate for anti-cancer therapy.
Full Name
L1 cell adhesion molecule
References
- Meier F, et al. (2006) The adhesion molecule L1 (CD171) promotes melanoma progression. Int J Cancer. 119(3): 549-55.
- Gavert N, et al. (2008) L1-CAM in cancerous tissues. Expert Opin Biol Ther. 8(11): 1749-57.
- Issa Y, et al. (2009) Enhanced L1CAM expression on pancreatic tumor endothelium mediates selective tumor cell transmigration. J Mol Med. 87(1): 99-112.
- Weidle UH, et al. (2009) L1-CAM as a target for treatment of cancer with monoclonal antibodies. Anticancer Res. 29(12): 4919-31.
- Raveh S, et al. (2009) L1 cell adhesion molecule (L1CAM) in invasive tumors. Cancer Lett. 282(2): 137-45.
- Wolterink S, et al. (2010) Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer Res. 70(6): 2504-15.
- Diagnostic and prognostic value of serum L1-cell adhesion molecule in esophageal squamous cell carcinomaAuthorXu, YW;Hong, CQ;Wu, ZY;Peng, YH;Ran, LQ;Yang, SH;Huang, BS;Liang, XY;Chen, HL;Wu, JY;Xu, XE;Deng, JW;Zou, HY;Fang, WK;Li, EM;Xu, LY;Xie, JJ;Year2018JournalClin Res Hepatol GastroenterolApplicationELISA (serum sample)PubMed ID: 30219694FoldedExpand
- Circulating Levels of L1-cell Adhesion Molecule as a Serum Biomarker for Early Detection of Gastric Cancer and Esophagogastric Junction AdenocarcinomaAuthorXu, EML;Xu, YW;Xie, JJ;Year2020JournalJournal of CancerApplicationELISAFoldedExpand
If you couldn"t find the specific citation, you can:
- Find All Citations
- Submit a CitationGet a Publication Reward
- Leave Us a Message
品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
联络我们
