Sino Biological/PCSK9 ELISA Kit, Mouse/1/KIT50251
商品编号：
KIT50251
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
PCSK9 ELISA Kit, Mouse: General Information
Product name
PCSK9 ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
47.09 pg/mL
Assay range
125-8000 pg/mL
Specificity
Recognizes both recombinant and natural Mouse PCSK9
Interference
Preparations of mouse LDLR at 30 ng/mL in a mid-range recombinantmouse PCSK9 control were assayed for interference. No significant interference was observed.
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 496.66 | 986.39 | 2,040.14 |
| SD | 25.11 | 47.54 | 76.26 |
| CV(%) | 5.1% | 4.8% | 3.7% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 505.93 | 993.25 | 2,061.85 |
| SD | 36.93 | 56.17 | 152.15 |
| CV(%) | 7.3% | 5.7% | 7.4% |
Recovery
The recovery of Mouse PCSK9 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 77 |
| Range |
| 72-80% |
Linearity
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 106% |
| 1:4 | 87% |
| 1:8 | 77% |
Materials provided
1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Sample Dilution Buffer Concentrate 7. Color Reagent A8. Color Reagent B9. Stop Solution
Product overview
This PCSK9 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse PCSK9 protein in Serum . It contains recombinant Mouse PCSK9, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA
PCSK9 ELISA Kit, Mouse: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural mouse PCSK9. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
PCSK9 ELISA Kit, Mouse: Synonyms
AI415265 ELISA Kit, Mouse; AI747682 ELISA Kit, Mouse; FH3 ELISA Kit, Mouse; HCHOLA3 ELISA Kit, Mouse; Narc1 ELISA Kit, Mouse; PC9 ELISA Kit, Mouse
PCSK9 Background Information
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
Full Name
proprotein convertase subtilisin/kexin type 9
Research Areas
- Serine Proteases and Regulators
References
- Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933.
- Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498
- Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73.
- Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875.
- Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
- Di"ao Xinxuekang Capsule, a Chinese Medicinal Product, Decreases Serum Lipids Levels in High-Fat Diet-Fed ApoE–/–Mice by Downregulating PCSK9AuthorQu, L;Li, D;Gao, X;Li, Y;Wu, J;Zou, W;Year2018JournalFrontiers in pharmacologyApplicationELISA (serum)PubMed ID: 30443213FoldedExpand
- A small-molecule inhibitor of PCSK9 transcription ameliorates atherosclerosis through the modulation of FoxO1/3 and HNF1αAuthorWang, X;Chen, X;Zhang, X;Su, C;Yang, M;He, W;Du, Y;Si, S;Wang, L;Hong, B;Year2020JournalEBioMedicineApplicationELISAFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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