Sino Biological/IL-6 ELISA Kit, Human/1/KIT10395A
商品编号：
KIT10395A
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IL-6 ELISA Kit, Human: General Information
Product name
IL-6 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum,Cell supernatant
Sensitivity
1.24 pg/mL
Assay range
5.47-350 pg/mL
Specificity
Recognizes both recombinant and natural Human IL-6
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IL6 control were assayed for interference. No significant interference was observed.
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 66.00 | 126.00 | 230.00 |
| SD | 1.82 | 5.94 | 17.09 |
| CV(%) | 2.7% | 4.7% | 7.4% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 59.00 | 116.00 | 215.00 |
| SD | 7.51 | 10.02 | 18.03 |
| CV(%) | 12.8% | 8.6% | 8.4% |
Recovery
The recovery of Human IL6 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 75 |
| Range |
| 72-78% |
| Sample |
| supernatant (n=3) |
| Average % Recovery |
| 110 |
| Range |
| 97-117% |
Linearity
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 96% |
| 1:4 | 104% |
| 1:8 | 98% |
| 1:16 | 98% |
Materials provided
1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution
Product overview
This IL-6 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-6 protein in Serum,Cell supernatant . It contains recombinant Human IL-6, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA
IL-6 ELISA Kit, Human: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL6. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL-6 ELISA Kit, Human: Synonyms
BSF2 ELISA Kit, Human; HGF ELISA Kit, Human; HSF ELISA Kit, Human; IFNB2 ELISA Kit, Human; IL-6 ELISA Kit, Human; Interleukin-6 ELISA Kit, Human
IL-6 Background Information
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
interleukin 6
Research Areas
- Neuroinflammation
- Cancer Drug Targets
Related Pathways
IL17 signaling pathway
References
- Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
- Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
- Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
- LncRNA-MEG3 alleviates high glucose induced inflammation and apoptosis of retina epithelial cells via regulating miR-34a/SIRT1 axisAuthorTong, P;Peng, QH;Gu, LM;Xie, WW;Li, WJ;Year2018JournalExp. Mol. Pathol.PubMed ID: 30529346FoldedExpand
- Colon Cancer Cell Secretes EGF to Promote M2 Polarization of TAM Through EGFR/PI3K/AKT/mTOR PathwayAuthorLian, G;Chen, S;Ouyang, M;Li, F;Chen, L;Yang, J;Year2019JournalTechnol. Cancer Res. Treat.ApplicationELISAPubMed ID: 31088266FoldedExpand
- AEBP1 Promotes the Occurrence and Development of Abdominal Aortic Aneurysm by Modulating Inflammation via the NF-κB PathwayAuthorRen, J;Han, Y;Ren, T;Fang, H;Xu, X;Lun, Y;Jiang, H;Xin, S;Zhang, J;Year2019JournalJ. Atheroscler. Thromb.ApplicationELISAPubMed ID: 31462616FoldedExpand
- Overexpression Of hsa-miR-664a-3p Is Associated With Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease Via Targeting FHL1AuthorZhong, S;Chen, C;Liu, N;Yang, L;Hu, Z;Duan, P;Shuai, D;Zhang, Q;Wang, Y;Year2019JournalInt J Chron Obstruct Pulmon DisApplicationELISAPubMed ID: 31632001FoldedExpand
- The association between Interleukin-6 rs1800795/rs1800797 polymorphisms and risk of rotator cuff tear in a Chinese populationAuthorLi, J;Jiang, L;Zhou, X;Wu, L;Li, D;Chen, G;Year2020JournalBiosci. Rep.ApplicationelisaPubMed ID: 32270859FoldedExpand
- Combined Amelioration of Prebiotic Resveratrol and Probiotic Bifidobacteria on Obesity and Nonalcoholic Fatty Liver DiseaseAuthorHu, D;Yang, W;Mao, P;Cheng, M;Year2020JournalNutr CancerPubMed ID: 32436410FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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