Sino Biological/IL-10 ELISA Kit, Human/1/KIT10947A
商品编号：
KIT10947A
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
IL-10 ELISA Kit, Human: General Information
Product name
IL-10 ELISA Kit, Human
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Cell supernatant
Sensitivity
7.26 pg/mL
Assay range
18.75-1200 pg/mL
Specificity
Recognizes both recombinant and natural Human IL-10
Interference
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human IL10 control were assayed for interference. No significant interference was observed.
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 109.00 | 230.00 | 480.00 |
| SD | 3.70 | 6.59 | 13.78 |
| CV(%) | 3.4% | 2.9% | 2.9% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 102.00 | 233.00 | 471.00 |
| SD | 5.86 | 6.08 | 11.37 |
| CV(%) | 5.7% | 2.6% | 2.4% |
Recovery
The recovery of Human IL10 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| supernatant (n=3) |
| Average % Recovery |
| 71 |
| Range |
| 70 -72% |
Linearity
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 100% |
| 1:4 | 100% |
| 1:8 | 107% |
| 1:16 | 99% |
Materials provided
1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution
Product overview
This IL-10 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-10 protein in Cell supernatant . It contains recombinant Human IL-10, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA
IL-10 ELISA Kit, Human: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human IL10. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL-10 ELISA Kit, Human: Synonyms
CSIF ELISA Kit, Human; GVHDS ELISA Kit, Human; IL-10 ELISA Kit, Human; IL10A ELISA Kit, Human; Interleukin-10 ELISA Kit, Human; TGIF ELISA Kit, Human
IL-10 Background Information
IL-10 is an anti-inflammatory cytokine that belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells, and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn"s disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of some cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen-presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
interleukin 10
Research Areas
- Neuroinflammation
References
- Arimoto T, et al. (2007) Interleukin-10 protects against inflammation-mediated degeneration of dopaminergic neurons in substantia nigra. Neurobiol Aging. 28(6):894-906.
- Han X, et al. (2010) Effect of cobalt protoporphyrin on hyperexpression of heme oxygenase-1 and secretion of IL-10 in rat bone marrow mesenchymal stem cells. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 18(5):1297-301.
- Cui QQ, et al. (2011) Expression of RhoA in the lung tissue of acute lung injury rats and the influence of RhoA on the expression of IL-8 and IL-10. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 77(7): 1436-41.
- Influence of Th17/Treg balance on progression of liver cirrhosis in patients with chronic hepatitis BAuthorWu, C;Dong, X;Yang, Q;Sun, Z;Year2018JournalInternational Journal of Clinical and Experimental MedicineApplicationELISAPubMed ID: 22745730FoldedExpand
- Colon Cancer Cell Secretes EGF to Promote M2 Polarization of TAM Through EGFR/PI3K/AKT/mTOR PathwayAuthorLian, G;Chen, S;Ouyang, M;Li, F;Chen, L;Yang, J;Year2019JournalTechnol. Cancer Res. Treat.PubMed ID: 31088266FoldedExpand
- A controllable, centrifugal-based hydrodynamic microfluidic chip for cell-pairing and studying long-term communications between single cellsAuthorLi, L;Wang, H;Huang, L;Michael, SA;Huang, W;Wu, H;Year2019JournalAnal. Chem.PubMed ID: 31741379FoldedExpand
- 透析方式与维持透析患者生活质量疗效和微炎症指标的相关性Author麻, 冰;虞, 妙;马, 丽;何, 帆;杨, 文;Year2020Journal河北医学FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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