Sino Biological/DKK3 ELISA Kit, Mouse/1/KIT50247
商品编号：
KIT50247
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
DKK3 ELISA Kit, Mouse: General Information
Product name
DKK3 ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
4.62 pg/mL
Assay range
12.5-800 pg/mL
Specificity
Recognizes both recombinant and natural Mouse DKK3
Precision
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 132.00 | 243.00 | 481.00 |
| SD | 7.98 | 7.82 | 12.03 |
| CV(%) | 6.0% | 3.2% | 2.5% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 132.00 | 262.00 | 495.00 |
| SD | 4.51 | 19.00 | 29.02 |
| CV(%) | 3.4% | 7.3% | 5.9% |
Recovery
The recovery of Mouse DKK3 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 83 |
| Range |
| 82 -84% |
Linearity
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 95% |
| 1:4 | 103% |
| 1:8 | 99% |
| 1:16 | 101% |
Materials provided
1. 96 well microplate coated with Capture Antibody2. Detection Antibody conjugated to HRP3. Standards4. Wash Buffer Concentrate5. Dilution Buffer Concentrate6. Color Reagent A7. Color Reagent B8. Stop Solution
Product overview
This DKK3 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse DKK3 protein in Serum . It contains recombinant Mouse DKK3, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃Opened/Reconstituted Reagents: Please refer to CoA
DKK3 ELISA Kit, Mouse: Images
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
DKK3 ELISA Kit, Mouse: Synonyms
AW061014 ELISA Kit, Mouse; C87148 ELISA Kit, Mouse
DKK3 Background Information
DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.
Full Name
dickkopf WNT signaling pathway inhibitor 3
Related Pathways
Canonical Wnt Pathway
References
- Yue W, et al., 2008. Downregulation of Dkk3 activates beta-catenin/TCF-4 signaling in lung cancer. Carcinogenesis, 29 (1): 84-92.
- Ding Z, et al., 2009. Promoter methylation and mRNA expression of DKK3 and WIF-1 in hepatocellular carcinoma. World J Gastroenterol, 15 (21):2595-601.
- DKK3 (Dickkopf 3) Alters Atherosclerotic Plaque Phenotype Involving Vascular Progenitor and Fibroblast Differentiation Into Smooth Muscle CellsAuthorKaramariti, E;Zhai, C;Yu, B;Lei, Q;Wang, Z;Potter, CMF;Wong, MM;Simpson, RML;Zhang, Z;Wang, X;Del Barco Barrantes, I;Niehrs, C;Kong, D;Zhao, Q;Zhang, Y;Hu, Y;Zhang, C;Xu, Q;Year2017JournalArterioscler. Thromb. Vasc. Biol.ApplicationELISA (serum)PubMed ID: 29284609FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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