Sino Biological/HRG/HPRG Matched ELISA Antibody Pair Set,Human/1/SEK10836
商品编号：
SEK10836
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HRG/HPRG Matched ELISA Antibody Pair Set,Human General Information
Product name
HRG/HPRG Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human HRG/HPRG
Assay range
15.63-1000 pg/mL
Materials provided
1. Capture Antibody 1 mg/mL of mouse anti-Human HRG/HPRG monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10836-MM01)2. Detection Antibody 0.2 mg/mL of rabbit anti-Human HRG/HPRG polyclonalantibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.4 μg/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 35 ng of recombinant Human HRG/HPRG. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
Product overview
This HRG/HPRG Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human HRG/HPRG . It contains Human HRG/HPRG capture antibody, Human HRG/HPRG detector antibody (HRP)and a highly purifiedHEK293-expressedrecombinant Human HRG/HPRG protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
HRG/HPRG Matched ELISA Antibody Pair Set,Human Images
HRG/HPRG Matched ELISA Antibody Pair Set,Human: Synonyms
HPRG Matched ELISA Antibody Pair Set, Human; HRGP Matched ELISA Antibody Pair Set, Human; THPH11 Matched ELISA Antibody Pair Set, Human
HRG/HPRG Background Information
Histidine-rich glycoprotein, also known as HRG and HPRG, is a glycoprotein located in plasma and platelets and contains an unusually large amount of histidine and proline. In humans, five distinct domains are recognized in the mature HPRG molecule. There are two N-terminal cystatin-like modules (aa 19 - 254) and one His-Pro-rich region (aa 350 - 497) that is flanked by two Pro-rich segments (aa 276 - 321 and 498 - 525). The His-Pro-rich region contains 10 tandem repeats with an HHPHG motif, and the N- and C-termini are linked by a disulfide bond. The specific functions of HRG remain unclear, but it is known that the protein binds heme, dyes, and divalent metal ions. It inhibits rosette formation and interacts with heparin, thrombospondin, and plasminogen. Two of the protein"s effects, the inhibition of fibrinolysis, and the reduction of inhibition of coagulation indicate a potential prothrombotic effect. HPRG is evolutionarily, functionally, and structurally related to cleaved high molecular weight kininogen (HKa), an anti-angiogenic polypeptide that stimulates apoptosis of proliferating endothelial cells through binding to cell-surface tropomyosin. The antiangiogenic activity of the multidomain plasma protein HPRG is localized to its histidine-proline-rich (H/P) domain and has recently been shown to be mediated, at least partially, through binding to cell-surface tropomyosin in fibroblast growth factor-2-activated endothelial cells.
Full Name
histidine-rich glycoprotein
References
- Guan X, et al. (2004) Histidine-proline rich glycoprotein (HPRG) binds and transduces anti-angiogenic signals through cell surface tropomyosin on endothelial cells. Thromb Haemost. 92(2): 403-12.
- Doate F, et al. (2004) Peptides derived from the histidine-proline domain of the histidine-proline-rich glycoprotein bind to tropomyosin and have antiangiogenic and antitumor activities. Cancer Res. 64(16): 5812-7.
- From Diatoms to Malaria: Synthesis and Application of Functional MaterialsAuthorSwartz, JD;Year2011JournalThesisFoldedExpand
- The nuclear bile acid receptor FXR controls the liver derived tumor suppressor histidine-rich glycoproteinAuthorDeuschle, U;Birkel, M;Hambruch, E;Hornberger, M;Kinzel, O;Perović-Ottstadt, S;Schulz, A;Hahn, U;Burnet, M;Kremoser, C;Year2014JournalInt. J. CancerApplicationELISA (plasma sample)PubMed ID: 25363753FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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