Sino Biological/CTLA-4蛋白，人，重组（His&；hFc标签）/1/11159-H03H
商品编号：
11159-H03H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
CTLA-4 Protein, Human, Recombinant (His & hFc Tag): Product Information
Purity
> 92 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.2-1μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80.
Protein Construction
A DNA sequence encoding the human CTLA4 (NP_005205.2) extracellular domain (Met 1-Phe 162) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Accession#
NP_005205.2
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Lys 36
Molecule Mass
The recombinant human CTLA4/Fc is a disulfide-linked homodimer. The reduced monomer consists of 375 amino acids and has a predicted molecular mass of 41.8 kDa. As a result of glycosylation, the apparent molecular mass of rh CTLA4/Fc monomer is approximately 50-55 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CTLA-4 Protein, Human, Recombinant (His & hFc Tag): Images
Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.2-1μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80.
CTLA-4 Protein, Human, Recombinant (His & hFc Tag): Synonyms
ALPS5 Protein, Human; CD Protein, Human; CD152 Protein, Human; CELIAC3 Protein, Human; CTLA-4 Protein, Human; GRD4 Protein, Human; GSE Protein, Human; IDDM12 Protein, Human
CTLA-4 Background Information
Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).
- Cancer Immunotherapy
- Co-inhibitory Immune Checkpoint Targets
- CTLA4 / CD152 Immune Checkpoint Proteins
- Immune Checkpoint
- Immune Checkpoint Detection: Antibodies
- Immune Checkpoint Detection: ELISA Antibodies
- Immune Checkpoint Detection: IP Antibodies
- Immune Checkpoint Detection: WB Antibodies
- Immune Checkpoint Proteins
- Immune Checkpoint Targets
- Immunotherapy
- Targeted Therapy
Full Name
cytotoxic T-lymphocyte-associated protein 4
Research Areas
- ITIM/ITAM Immunoreceptors and Related Molecules
- Cancer Drug Targets
References
- Slavik JM, et al. (1999) CD28/CTLA-4 and CD80/CD86 families: signaling and function. Immunol Res. 19(1): 1-24.
- Holmberg D, et al. (2005) CTLA-4 (CD152) and its involvement in autoimmune disease. Autoimmunity. 38(3): 225-33.
- Chin LT, et al. (2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases. Chang Gung Med J. 31(1): 1-15.
Human CTLA-4 Proteins
- CTLA-4 Protein, Human, Recombinant (His Tag)11159-H08H
- CTLA-4 Protein, Human, Recombinant (His Tag), Biotinylated11159-H08H-B
- CTLA-4 Protein, Human, Recombinant11159-HNAH
- CTLA-4 Protein, Human, Recombinant (hFc Tag)11159-H02H6
- CTLA-4 Protein, Human, Recombinant (hFc Tag), Biotinylated11159-H02H6-B
- CTLA-4 Protein, Human, Recombinant (rFc Tag)11159-H31H5
Mouse CTLA-4 Proteins
- CTLA-4 Protein, Mouse, Recombinant (His Tag), Biotinylated50503-M08H-B
- CTLA-4 Protein, Mouse, Recombinant (His Tag), HPLC-verifiedHPLC-50503-M08H
- CTLA-4 Protein, Mouse, Recombinant (hFc Tag)50503-M02H
- CTLA-4 Protein, Mouse, Recombinant (ECD, His Tag)50503-M08H
Rat CTLA-4 Proteins
- CTLA-4 Protein, Rat, Recombinant (ECD, His Tag)81069-R08H
- CTLA-4 Protein, Rat, Recombinant (ECD, hFc Tag)81069-R02H
Cynomolgus, Rhesus CTLA-4 Proteins
- CTLA-4 Protein, Cynomolgus, Rhesus, Recombinant (hFc Tag)90213-C02H
- Cynomolgus / Rhesus CTLA4 / CD152 Protein (His Tag)90213-C08H
Canine CTLA-4 Proteins
- CTLA-4 Protein, Canine, Recombinant (ECD, His Tag)70113-D08H
- CTLA-4 Protein, Canine, Recombinant (ECD, hFc Tag)70113-D02H
- Isolation of Two Novel Human Anti-CTLA-4 mAbs with Intriguing Biological Properties on Tumor and NK CellsAuthorPassariello, M;Vetrei, C;Sasso, E;Froechlich, G;Gentile, C;D"Alise, AM;Zambrano, N;Scarselli, E;Nicosia, A;De Lorenzo, C;Year2020JournalCancers (Basel)ApplicationbiopannningPubMed ID: 32781690FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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