Sino Biological/KEAP1 Protein, Human, Recombinant/1/11981-HNCB
商品编号：
11981-HNCB
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
KEAP1 Protein, Human, Recombinant: Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human KEAP1 (Q14145) (Gln2-Cys624) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Accession#
Q14145
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Gly
Molecule Mass
The secreted recombinant human KEAP1 consists of 625 amino acids and predicts a molecular mass of 69.7 KDa. The apparent molecular mass of the protein is approximately 64 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
KEAP1 Protein, Human, Recombinant: Images
KEAP1 Protein, Human, Recombinant: Synonyms
INRF2 Protein, Human; KEAP-1 Protein, Human; KLHL19 Protein, Human
KEAP1 Background Information
Kelch-like ECH-associated protein 1, also known as a cytosolic inhibitor of Nrf2, Kelch-like protein 19, KEAP1, and INRF2, is a cytoplasm and nucleus protein that contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain, and six Kelch repeats. KEAP1 / INRF2 is broadly expressed, with the highest levels in skeletal muscle. KEAP1 / INRF2 is a key regulator of the NRF2 transcription factor, which transactivates the antioxidant response element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1 / INRF2 targets NRF2 for ubiquitination and proteolytic degradation and as such is responsible for the rapid turnover of NRF2. KEAP1 / INRF2 retains NFE2L2 / NRF2 in the cytosol. KEAP1 / INRF2 functions as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. It targets NFE2L2 / NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. KEAP1 / INRF2 may also retain BPTF in the cytosol. It targets PGAM5 for ubiquitination and degradation by the proteasome.
Full Name
kelch like ECH associated protein 1
References
- Strachan GD. et al., 2004, Biochemistry. 43: 12113-22.
- Zhang DD. et al., 2004, Mol Cell Biol. 24: 10941-53.
- Zhang DD. et al., 2003, Mol Cell Biol. 23: 8137-51.
- Lo S-C. et al., 2006, J Biol Chem. 281: 37893-903.
- Buckley BJ. et al., 2008, Free Radic Biol Med. 44 (4) :692-8.
Human KEAP1 Proteins
- KEAP1 Protein, Human, Recombinant (His & GST Tag)11981-H20B
- KEAP1 Protein, Human, Recombinant (His & GST & AVI Tag)11981-H29B
- GULP1 regulates the NRF2-KEAP1 signaling axis in urothelial carcinomaAuthorHayashi, M;Guida, E;Inokawa, Y;Goldberg, R;Reis, LO;Ooki, A;Pilli, M;Sadhukhan, P;Woo, J;Choi, W;Izumchenko, E;Gonzalez, LM;Marchionni, L;Zhavoronkov, A;Brait, M;Bivalacqua, T;Baras, A;Netto, GJ;Koch, W;Singh, A;Hoque, MO;Year2020JournalSci SignalApplicationbindingPubMed ID: 32817372FoldedExpand
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Mammalian transient protein expression service
Baculovirus-insect expression service
Stable cell line development service (CHO / HEK293)
品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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