Sino Biological/NKG2D Protein, Human, Recombinant (aa 78-216, His Tag)/1/10575-H07B
商品编号：
10575-H07B
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
NKG2D Protein, Human, Recombinant (aa 78-216, His Tag): Product Information
Purity
> 90 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
1. Immobilized human His-NKG2D (78-216) at 2 μg/ml (100 μl/well) can bind human ULBP1-Fch (Cat: 10679-H03H), The EC50 of human ULBP1-Fch (Cat: 10679-H03H) is 8-50 ng/mL2. Immobilized human His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch (Cat: 10759-H03H), The EC50 of human MICB-Fch (Cat: 10759-H03H) is 15-60 ng/mL.
Protein Construction
A DNA sequence encoding the human NKG2D (P26718-1) (Phe78-Val216) was fused with a polyhistide tag at the N-terminus.
Accession#
P26718-1
Accession#
NP_031386.2
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
His
Molecule Mass
The recombinant human NKG2D consists of 157 amino acids and has a calculated molecular mass of 18.4 kDa. As a result of glycosylation, the apparent molecular mass of human NKG2D is approximately 22.6 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20mM Tris, 300mM NaCl, 10%glycerol, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
NKG2D Protein, Human, Recombinant (aa 78-216, His Tag): Images
Immobilized human His-NKG2D (78-216) at 2 μg/ml (100 μl/well) can bind human ULBP1-Fch (Cat: 10679-H03H), The EC50 of human ULBP1-Fch (Cat: 10679-H03H) is 8-50 ng/mL.
Immobilized human His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch (Cat: 10759-H03H), The EC50 of human MICB-Fch (Cat: 10759-H03H) is 15-60 ng/mL.
NKG2D Protein, Human, Recombinant (aa 78-216, His Tag): Synonyms
CD314 Protein, Human; D12S2489E Protein, Human; KLR Protein, Human; NKG2-D Protein, Human; NKG2D Protein, Human
NKG2D Background Information
KLRK1 (Killer Cell Lectin Like Receptor K1) is a Protein Coding gene. NKG2D, also known as CD314, is an immune receptor that consists of two disulfide-linked type II transmembrane proteins with short intracellular proteins incapable to transduce signals. To transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.
Full Name
killer cell lectin-like receptor subfamily K, member 1
References
- Houchins J, et al. (1991) DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells. J Exp Med. 173: 1017-102.
- Bauer S, et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285(5428):727-9.
- Zafirova B, et al. (2011) Regulation of immune cell function and differentiation by the NKG2D receptor. Cell Mol Life Sci. 68(21):3519-29.
Human NKG2D Proteins
- NKG2D Protein, Human, Recombinant (hFc Tag)10575-H01S
Mouse NKG2D Proteins
- NKG2D Protein, Mouse, Recombinant (hFc Tag)57340-M01B
Rhesus NKG2D Proteins
- NKG2D Protein, Rhesus, Recombinant (aa 78-216, His Tag)90164-C07B
- Biophysical and biochemical characterization of a VHH-based IgG-like bi- and trispecific antibody platformAuthorPekar, L;Busch, M;Valldorf, B;Hinz, S;Toleikis, L;Krah, S;Zielonka, S;Year2020JournalmAbsApplicationoctetFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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