Sino Biological/c-MET Protein, Mouse, Recombinant (hFc Tag)/1/50622-M02H
商品编号：
50622-M02H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
c-MET Protein, Mouse, Recombinant (hFc Tag): Product Information
Purity
> 92 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized human HGF at 2 μg/ml (100 μl/well) can bind mouse HGFR with a linear range of 1.28-32ng/ml.
Protein Construction
A DNA sequence encoding the mouse MET (NP_032617.2) extracellular domain (Met 1-Asn 929) was fused with the Fc region of human IgG1 at the C-terminus.
Accession#
NP_032617.2
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Glu 25 & Ser 307
Molecule Mass
The recombinant mouse Met/Fc chimera is a disulfide-linked homodimer of the Met which is a heterodimer composed of the proteolytically cleaved α and β subunits. Each α and β together with the C-terminal Fc tag consists of 1146 amino acids and has a predicted molecular mass of 128 (α =32 + Fc tagged β=96) kDa. The apparent molecular mass of the rm MET/Fc heterodimer thus is approximately 43 kDa and 115-120 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
c-MET Protein, Mouse, Recombinant (hFc Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized human HGF at 2 μg/ml (100 μl/well) can bind mouse HGFR with a linear ranger of 1.28-32ng/ml.
c-MET Protein, Mouse, Recombinant (hFc Tag): Synonyms
AI838057 Protein, Mouse; c-Met Protein, Mouse; HGF Protein, Mouse; HGFR Protein, Mouse; Par4 Protein, Mouse
c-MET Background Information
Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that is overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair, or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.
- Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
Full Name
MET proto-oncogene, receptor tyrosine kinase
Research Areas
- Cancer Drug Targets
- Receptor Tyrosine Kinases (RTKs)
References
- McGill GG, et al. (2006) c-Met expression is regulated by Mitf in the melanocyte lineage. J Biol Chem. 281(15): 10365-73.
- Garcia S, et al. (2007) c-Met overexpression in inflammatory breast carcinomas: automated quantification on tissue microarrays. British journal of cancer. 96(2): 329-35.
- Socoteanu MP, et al. (2008) c-Met targeted therapy of cholangiocarcinoma. World J Gastroenterol. 14(19): 2990-4.
- Kong DS, et al. (2009) Prognostic significance of c-Met expression in glioblastomas. Cancer. 115(1): 140-8.
Human c-MET Proteins
- c-MET Protein, Human, Recombinant (ECD, His & AVI Tag), Biotinylated10692-H27H-B
- c-MET Protein, Human, Recombinant (ECD, hFc Tag)10692-H02H
- c-MET Protein, Human, Recombinant (aa 956-1390, His & GST Tag)10692-H20B1
- c-MET Protein, Human, Recombinant (ECD, His & hFc Tag)10692-H03H
- c-MET Protein, Human, Recombinant (His & hFc Tag), Biotinylated10692-H03H-B
- c-MET Protein, Human, Recombinant (ECD, His Tag)10692-H08H
Mouse c-MET Proteins
- c-MET Protein, Mouse, Recombinant (His Tag)50622-M08H
Rat c-MET Proteins
- c-MET Protein, Rat, Recombinant (hFc Tag)80004-R02H
Canine c-MET Proteins
- c-MET Protein, Canine, Recombinant (His Tag)70008-D08H
Cynomolgus, Rhesus c-MET Proteins
- c-MET Protein, Cynomolgus, Rhesus, Recombinant90304-CCCH
- c-MET Protein, Cynomolgus, Rhesus, Recombinant (His Tag)90304-C08H
- c-MET Protein, Cynomolgus, Rhesus, Recombinant (hFc Tag)90304-C02H
- A novel non-agonist c-Met antibody drug conjugate with superior potency over a c-Met tyrosine kinase inhibitor in c-Met amplified and non-amplified cancersAuthorFujita, R;Blot, V;Wong, E;Stewart, C;Lieuw, V;Richardson, R;Banah, A;Villicana, J;Timmer, A;Coronella, J;Newman, R;Gymnopoulos, M;Year2020JournalCancer Biol. Ther.PubMed ID: 32192391FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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