Sino Biological/EphA2 Protein, Human, Recombinant (His Tag)/1/13926-H08H
商品编号：
13926-H08H
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
EphA2 Protein, Human, Recombinant (His Tag): Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA. Immobilized human EPHA2-His at 10 μg/ml (100 μl/well) can bind human EFNA1-Fch (Cat:10882-H03H), The EC50 of human EFNA1-Fch (Cat:10882-H03H) is 0.1-0.2 μg/ml.
Protein Construction
A DNA sequence encoding the human EPHA2 (P29317) (Met1-Asn534) was expressed with a polyhistidine tag at the C-terminus.
Accession#
P29317
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ala 24
Molecule Mass
The recombinant human EPHA2 consists of 522 amino acids and predicts a molecular mass of 57.6 KDa. It migrates as an approximately 65-75 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.5.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EphA2 Protein, Human, Recombinant (His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized human EPHA2-His at 10 μg/ml (100 μl/well) can bind human EFNA1-Fch (Cat: 10882-H03H), The EC50 of human EFNA1-Fch (Cat: 10882-H03H) is 0.1-0.2 μg/ml.
EphA2 Protein, Human, Recombinant (His Tag): Synonyms
ARCC2 Protein, Human; CTPA Protein, Human; CTPP1 Protein, Human; CTRCT6 Protein, Human; ECK Protein, Human
EphA2 Background Information
Eph receptor A2 (Ephrin type-A receptor 2 or EphA2) is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph receptors" corresponding family of ligands are the ephrins anchored to cell surfaces. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps. They have also been found implicated in embryonic patterning, neuronal targeting, vascular development and adult neovascularization. The large family of ligands and receptors may make a major contribution to the accurate spatial patterning of connections and cell position in the nervous system. Furthermore, elevated expression of Eph receptors and ephrin ligands is associated with tumors and associated tumor vasculature, suggesting the Eph receptors and ephrin ligands also play critical roles in tumor angiogenesis and tumor growth. Unlike most Eph kinases, which are primarily expressed during development, EphA2 is primarily found in adult human epithelial cells. The cellular functions of EphA2 may be regulating cell growth, survival, migration, and angiogenesis.Unlike other receptor tyrosine kinases, ligand binding is not necessary for EphA2. Rather, the ligand appears to regulate EphA2 subcellular localization and its interactions with downstream adapter and signaling proteins. Eph receptor A2(EphA2) has been demonstrated to critically regulate tumor cell growth, migration and invasiveness. Eph receptor A2(EphA2) is frequently overexpressed and functionally altered in aggressive tumor cells, and that these changes promote metastatic character.
Full Name
EPH receptor A2
Related Pathways
Actin Dynamics Signaling Pathway
G Protein-coupled Receptors Signaling
MAPK-Erk Pathway
Autophagy Pathway
References
- Flanagan JG, et al. (1998) The ephrins and Eph receptors in neural development. Annu Rev Neurosci. 21: 309-45.
- Cheng N, et al. (2002) The ephrins and Eph receptors in angiogenesis. Cytokine Growth Factor Rev. 13(1): 75-85.
- Pratt RL, et al. (2002) Activation of the EphA2 tyrosine kinase stimulates the MAP/ERK kinase signaling cascade. Oncogene. 21(50): 7690-9.
- Jennifer Walker-Daniels, et al. (2003) Differential Regulation of EphA2 in Normal and Malignant Cells. Am J Pathol. 162(4): 1037-1042.
Human EphA2 Proteins
- EphA2 Protein, Human, Recombinant (aa 585-976, His & GST Tag)13926-H20B1
- EphA2 Protein, Human, Recombinant (ECD, His & AVI Tag), Biotinylated13926-H27H-B
Mouse EphA2 Proteins
- EphA2 Protein, Mouse, Recombinant (His Tag)50586-M08H
- Drug Conjugates for Targeting Eph Receptors in GlioblastomaAuthorSharma, P;Roberts, C;Herpai, D;Fokt, I;Priebe, W;Debinski, W;Year2020JournalPharmaceuticalsFoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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