Sino Biological/CD27 Protein, Human, Recombinant (His Tag)/1/10039-H08B1
商品编号：
10039-H08B1
品牌：
sinobiological
市场价：
¥0.00
美元价：
0.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
CD27 Protein, Human, Recombinant (His Tag): Product Information
Purity
> 85 % as determined by SDS-PAGE
Endotoxin
< 1.0="" eu="" per="" μg="" of="" the="" protein="" as="" determined="" by="" the="" lal="">
Activity
Measured by its binding ability in a functional ELISA.Immobilized human CD27-his (Cat:10039-H08B1) at 10 μg/mL (100 μl/well) can bind biotinylated human CD70-Fc (Cat:10780-H01H), The EC50 of biotinylated human CD70-Fc (Cat:10780-H01H) is 57-85 ng/mL.
Protein Construction
A DNA sequence encoding the human CD27(P26842) ( Met 1-Ile192) was expressed with a C-terminal polyhistidine tag.
Accession#
P26842
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Ala 20
Molecule Mass
The secreted recombinant human CD27 consists of 183 amino acids and predicts a molecular mass of 20.7 KDa. The apparent molecular mass of the protein is approximately 27 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
CD27 Protein, Human, Recombinant (His Tag): Images
Measured by its binding ability in a functional ELISA. Immobilized human CD27-his (Cat: 10039-H08B1) at 10 μg/mL (100 μl/well) can bind biotinylated human CD70-Fc (Cat: 10780-H01H), The EC50 of biotinylated human CD70-Fc (Cat: 10780-H01H) is 57-85 ng/mL.
CD27 Protein, Human, Recombinant (His Tag): Synonyms
S152 Protein, Human; S152. LPFS2 Protein, Human; S152.LPFS2 Protein, Human; T14 Protein, Human; TNFRSF7 Protein, Human; Tp55 Protein, Human
CD27 Background Information
CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.
- Cancer Immunotherapy
- Co-stimulatory Immune Checkpoint Targets
- Immune Checkpoint
- Immune Checkpoint Detection: Antibodies
- Immune Checkpoint Detection: ELISA Antibodies
- Immune Checkpoint Detection: FCM Antibodies
- Immune Checkpoint Detection: ICC Antibodies
- Immune Checkpoint Detection: IHC Antibodies
- Immune Checkpoint Proteins
- Immune Checkpoint Targets
- Immunotherapy
- Targeted Therapy
Full Name
CD27 molecule
Research Areas
- Cancer Drug Targets
References
- Drner T, et al. (2004) Correlation of circulating CD27 high plasma cells and disease activity in systemic lupus erythematosus. Lupus. 13(5): 283-9.
- Sahota SS, et al. (2009) CD27 in defining memory B-cell origins in Waldenstrm"s macroglobulinemia. Clin Lymphoma Myeloma. 9(1): 33-5.
- Jiang J, et al. (2010) Reduced CD27 expression on antigen-specific CD4+ T cells correlates with persistent active tuberculosis. J Clin Immunol. 30(4): 566-73.
Human CD27 Proteins
- CD27 Protein, Human, Recombinant (His Tag), Biotinylated10039-H08B1-B
- CD27 Protein, Human, Recombinant (rFc Tag)10039-H31H
- CD27 Protein, Human, Recombinant (His & hFc Tag)10039-H03H
- CD27 Protein, Human, Recombinant (hFc & AVI Tag), Biotinylated10039-H41H-B
- CD27 Protein, Human, Recombinant (ECD, hFc Tag)10039-H02H
Mouse CD27 Proteins
- CD27 Protein, Mouse, Recombinant (His & hFc Tag)50110-M03H
- CD27 Protein, Mouse, Recombinant (ECD, mFc Tag)50110-M38H
- CD27 Protein, Mouse, Recombinant (His Tag)50110-M08H
Rat CD27 Proteins
- CD27 Protein, Rat, Recombinant (hFc Tag)80223-R02H
- CD27 Protein, Rat, Recombinant (mFc Tag)80223-R38H
Rhesus CD27 Proteins
- CD27 Protein, Rhesus, Recombinant (hFc Tag)90049-C02H
- Development of homogeneous plasmonic potency assay using gold nanoparticle immunocomplexesAuthorHan, JH;Li, F;Gunawan, RC;Year2020JournalJ Pharm Biomed AnalApplicationmedical nanoparticle processPubMed ID: 31982688FoldedExpand
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品牌介绍
北京义翘神州科技股份有限公司（Sino Biological Inc.）是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务。公司目前生产和销售的现货产品种类超过 4.6 万种，其中重组蛋白约 6,000 种，包括超过 3,800 种人源细胞表达重组蛋白产品，能够全面满足客户对于最接近人体天然蛋白结构和性质的重组蛋白需求；公司还能提供约 12,000 种抗体，其中单克隆抗体数量约 4,600 种，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供「一站式」生物试剂产品和技术服务。义翘神州的客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲建立了子公司，已成为生物试剂行业国内领先的科技公司之一。
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